Abstract
Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.
| Original language | English |
|---|---|
| Pages (from-to) | 1300-1306 |
| Number of pages | 7 |
| Journal | Nature Genetics |
| Volume | 40 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2008 |
Bibliographical note
Funding Information:We thank D. Steger, D. Wykoff, A. Carroll and C. Hopkins from Agilent for advice regarding microarray, ChIP and other procedures; T. Lee and R. Young for sharing their tiling-array design and hybridization protocol before publication; H. Margalit and members of the O’Shea, Friedman and Regev laboratories for helpful discussions; and P. Grosu for help with Rosetta Resolver. We are also grateful to E. Lander, D. Pe’er, D. Koller, R. Losick, M. Brenner and B. Stern for reading the manuscript before publication. A.P.C. was a Howard Hughes Medical Institute (HHMI) Fellow of the Life Sciences Research Foundation and A.R. was supported by a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. This work was supported by HHMI (E.K.O.) and a grant from the Human Frontiers Science Program (E.K.O., A.R. and N.F.).