Structure and function of a transcriptional network activated by the MAPK Hog1

Andrew P. Capaldi, Tommy Kaplan, Ying Liu, Naomi Habib, Aviv Regev, Nir Friedman, Erin K. O’shea*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

182 Scopus citations

Abstract

Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.

Original languageAmerican English
Pages (from-to)1300-1306
Number of pages7
JournalNature Genetics
Volume40
Issue number11
DOIs
StatePublished - Nov 2008

Bibliographical note

Funding Information:
We thank D. Steger, D. Wykoff, A. Carroll and C. Hopkins from Agilent for advice regarding microarray, ChIP and other procedures; T. Lee and R. Young for sharing their tiling-array design and hybridization protocol before publication; H. Margalit and members of the O’Shea, Friedman and Regev laboratories for helpful discussions; and P. Grosu for help with Rosetta Resolver. We are also grateful to E. Lander, D. Pe’er, D. Koller, R. Losick, M. Brenner and B. Stern for reading the manuscript before publication. A.P.C. was a Howard Hughes Medical Institute (HHMI) Fellow of the Life Sciences Research Foundation and A.R. was supported by a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. This work was supported by HHMI (E.K.O.) and a grant from the Human Frontiers Science Program (E.K.O., A.R. and N.F.).

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