Structure of human IgM rheumatoid factor Fab bound to its autoantigen IgG Fc reveals a novel topology of antibody-antigen interaction

Adam L. Corper, Maninder K. Sohi, Vincent R. Bonagura, Michael Steinitz, Royston Jefferis, Arnold Feinstein, Dennis Beale, Michael J. Taussig, Brian J. Sutton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

202 Scopus citations

Abstract

Rheumatoid factors are the characteristic autoantibodies of rheumatoid arthritis, which bind to the Fc regions of IgG molecules. Here we report the crystal structure of the Fab fragment of a patient-derived IgM rheumatoid factor (RF-AN) complexed with human IgG4 Fc, at 3.2 Å resolution. This is the first structure of an autoantibody-autoantigen complex. The epitope recognized in IgG Fc includes the Cγ2/Cγ3 cleft region, and overlaps the binding sites of bacterial Fc-binding proteins. The antibody residues involved in autorecognition are all located at the edge of the conventional combining site surface, leaving much of the latter available, potentially, for recognition of a different antigen. Since an important contact residue is a somatic mutation, the structure implicates antigen-driven selection, following somatic mutation of germline genes, in the production of pathogenic rheumatoid factors.

Original languageEnglish
Pages (from-to)374-381
Number of pages8
JournalNature Structural Biology
Volume4
Issue number5
DOIs
StatePublished - May 1997

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