Structure of the Surface Glycoprotein from Halobacterium volcanii as Revealed by Electron Microscopy

M. Kessel, I. Wildhaber, S. Cohen, W. Baumeister, Uwe B. Sleytr (Editor), Paul Messner (Editor), Dietmar Pum (Editor), Margit Sára (Editor)

Research output: Contribution to conferencePaper

Abstract

The cell surfaces of Halobacteria are seen by shadow casting to be composed of a hexagonal lattice of morphological units with a centre-to-centre spacing of approximately 15 nm. Preparations of virtually salt-free envelopes from Halobacterium volcanii, isolated from the Dead Sea, can be obtained by freezing and thawing the cells, treatment with 1 μg/ml of DNAase and suspension in 10 mM CaCl2 and have enabled a three-dimensional analysis of the surface layer by negative staining.
Original languageAmerican English
Pages79-82
Number of pages4
StatePublished - 1988

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