Studies of inactivation, retardation and accumulation of viruses in porous media by a combination of dye labeled and native bacteriophage probes

Vitaly Gitis*, Christina Dlugy, Jenny Gun, Ovadia Lev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Penetration of viruses through soils is governed by the processes of transport, reversible adsorption, accumulation and inactivation. Until now, it was difficult to decouple the latter two processes and accurately predict viral fate. The present work describes a novel method-tracer studies with a mixture of native and fluorescent-dyed bacteriophages-that facilitates parallel quantification of the two processes. When the native phages are experiencing both accumulation and inactivation, the labeled ones are inactivated already and therefore can only be accumulated. Thus the effect of inactivation is applicable to native bacteriophages only and depletion of phage concentration due to inactivation can be elucidated from a total phage balance. The novel approach is exemplified by batch and column studies of the effects of temperature, pH, and saturation, on inactivation of MS2 bacteriophage. A three-parameter model accounting for inactivation, reversible adsorption (i.e., retardation), and accumulation is implemented.

Original languageAmerican English
Pages (from-to)43-49
Number of pages7
JournalJournal of Contaminant Hydrology
Volume124
Issue number1-4
DOIs
StatePublished - 1 Jun 2011

Bibliographical note

Funding Information:
This research was supported by the Israel Science Foundation (grant No. 1184/06 ). We thank Mrs. Lada Krementsky for her help with batch experiments.

Keywords

  • Attachment
  • Labeled bacteriophages
  • MS2
  • Residence time distribution
  • Subsoil

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