TY - JOUR
T1 - Studies on a naturally occurring human antibody active against mouse landschutz ascites tumor cells
T2 - II. Identification of the antibody as igm and its partial purification
AU - Laskov, R.
AU - Simon, E.
AU - Ram, D.
AU - Gross, J.
PY - 1968/1/15
Y1 - 1968/1/15
N2 - 1. The cytotoxic antibody in normal human serum, active against mouse tumor cells, was identified as a 19S macroglobulin by means of Sephadex gel filtration, DEAE‐cellulose chromatography and density‐gradient centrifugation procedures. 2. A purified IgM fraction was isolated from normal human serum. The cytotoxic activity of this fraction, as tested against mouse tumor cells, was enriched 20–80 times over that of the unfractionated serum. The purified IgM showed mainly one precipitin line on immunoelectrophoretic analysis. In the ultracentrifuge the 19S peak comprised about 85% of the total protein.
AB - 1. The cytotoxic antibody in normal human serum, active against mouse tumor cells, was identified as a 19S macroglobulin by means of Sephadex gel filtration, DEAE‐cellulose chromatography and density‐gradient centrifugation procedures. 2. A purified IgM fraction was isolated from normal human serum. The cytotoxic activity of this fraction, as tested against mouse tumor cells, was enriched 20–80 times over that of the unfractionated serum. The purified IgM showed mainly one precipitin line on immunoelectrophoretic analysis. In the ultracentrifuge the 19S peak comprised about 85% of the total protein.
UR - http://www.scopus.com/inward/record.url?scp=0014414765&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910030113
DO - 10.1002/ijc.2910030113
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 5656880
AN - SCOPUS:0014414765
SN - 0020-7136
VL - 3
SP - 107
EP - 115
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 1
ER -
