Studies on the biological role of DNA methylation: III. Role in excision of one-genome long single-stranded øX174 DNA

Joseph Friedman; Adam Friedmann; Aharon Razin

doi:10.1093/nar/4.10.3483

Studies on the biological role of DNA methylation: III. Role in excision of one-genome long single-stranded øX174 DNA

Joseph Friedman^*
, Adam Friedmann
, Aharon Razin

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Accumulation of replicative intermediates of the bacteriophage øX174 was observed in E.coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded "tails". These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is obligatory for the final maturation of the phage.

Original language	English
Pages (from-to)	3483-3496
Number of pages	14
Journal	Nucleic Acids Research
Volume	4
Issue number	10
DOIs	https://doi.org/10.1093/nar/4.10.3483
State	Published - Oct 1977

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title = "Studies on the biological role of DNA methylation: III. Role in excision of one-genome long single-stranded {\o}X174 DNA",

abstract = "Accumulation of replicative intermediates of the bacteriophage {\o}X174 was observed in E.coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded {"}tails{"}. These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is obligatory for the final maturation of the phage.",

author = "Joseph Friedman and Adam Friedmann and Aharon Razin",

year = "1977",

month = oct,

doi = "10.1093/nar/4.10.3483",

language = "אנגלית",

volume = "4",

pages = "3483--3496",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "10",

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T2 - III. Role in excision of one-genome long single-stranded øX174 DNA

AU - Friedman, Joseph

AU - Friedmann, Adam

AU - Razin, Aharon

PY - 1977/10

Y1 - 1977/10

N2 - Accumulation of replicative intermediates of the bacteriophage øX174 was observed in E.coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded "tails". These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is obligatory for the final maturation of the phage.

AB - Accumulation of replicative intermediates of the bacteriophage øX174 was observed in E.coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded "tails". These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is obligatory for the final maturation of the phage.

UR - https://www.scopus.com/pages/publications/0017754188

U2 - 10.1093/nar/4.10.3483

DO - 10.1093/nar/4.10.3483

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AN - SCOPUS:0017754188

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EP - 3496

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 10

ER -

Studies on the biological role of DNA methylation: III. Role in excision of one-genome long single-stranded øX174 DNA

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