Studies on the physiological manifestations of cell mediated cytotoxicity. II. Inhibition of [3H]thymidine incorporation by plasmacytoma cells exposed in vitro to sensitized splenocytes

M. Steinitz*, M. Feigis, D. W. Weiss

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Splenocytes of both allogeneic and syngeneic origin, derived from mice challenged with a mineral oil induced BALB/c plasmacytoma (PCT), effected a marked and rapid inhibition of tritiated thymidine incorporation by the PCT cells in vitro. This inhibition was evident in the syngeneic confrontation even though exposure to the sensitized effector cells did not lead to detectable release of 51Cr from labeled PCT target cells during 6 hr of incubation, a period of time sufficient to bring about appreciable chromium release when the spleen cells were allogeneic. The degree of inhibition of thymidine uptake caused by allogeneic effector cells was related to the effector/target cell ratio. Irradiation of the allogeneic splenocytes reduced or abolished their inhibitory capacity. Supernatants of interactions between PCT and sensitized allogeneic spleen cells had no specific inhibitory activity. It is suggested that inhibition of DNA precursor incorporation may offer a rapid and accurate means of assessing cytostatic effects mounted by sensitized effector cells.

Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalCellular Immunology
Volume17
Issue number1
DOIs
StatePublished - May 1975

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