Abstract
Splenocytes of both allogeneic and syngeneic origin, derived from mice challenged with a mineral oil induced BALB/c plasmacytoma (PCT), effected a marked and rapid inhibition of tritiated thymidine incorporation by the PCT cells in vitro. This inhibition was evident in the syngeneic confrontation even though exposure to the sensitized effector cells did not lead to detectable release of 51Cr from labeled PCT target cells during 6 hr of incubation, a period of time sufficient to bring about appreciable chromium release when the spleen cells were allogeneic. The degree of inhibition of thymidine uptake caused by allogeneic effector cells was related to the effector/target cell ratio. Irradiation of the allogeneic splenocytes reduced or abolished their inhibitory capacity. Supernatants of interactions between PCT and sensitized allogeneic spleen cells had no specific inhibitory activity. It is suggested that inhibition of DNA precursor incorporation may offer a rapid and accurate means of assessing cytostatic effects mounted by sensitized effector cells.
Original language | English |
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Pages (from-to) | 181-191 |
Number of pages | 11 |
Journal | Cellular Immunology |
Volume | 17 |
Issue number | 1 |
DOIs | |
State | Published - May 1975 |