TY - JOUR
T1 - Studies on the Specificity of the Interaction Between Peptidyl‐tRNA and E. Coli Ribosomes, Initiation and Elongation Factors
AU - Degroot, N.
AU - Panet, A.
AU - Lapidot, Y.
PY - 1972
Y1 - 1972
N2 - Several aspects of the binding of peptidyl‐tRNA to E. coli ribosomes were studied. The E. coli binding factor (EF1) does not increase the rate of peptidyl‐tRNA binding. From the results obtained it was concluded that two molecules of Gly2 Phe‐tRNA can bind to one and the same ribosome, but no new peptide bond was formed. Gly, Phe‐tRNA at the A site inhibited the puromycin reaction of Gly2 Phe‐tRNA at the P site of the same ribosome. The binding of peptidyl‐tRNA at the P site activates the adjacent A site for aminoacyl‐tRNA binding.
AB - Several aspects of the binding of peptidyl‐tRNA to E. coli ribosomes were studied. The E. coli binding factor (EF1) does not increase the rate of peptidyl‐tRNA binding. From the results obtained it was concluded that two molecules of Gly2 Phe‐tRNA can bind to one and the same ribosome, but no new peptide bond was formed. Gly, Phe‐tRNA at the A site inhibited the puromycin reaction of Gly2 Phe‐tRNA at the P site of the same ribosome. The binding of peptidyl‐tRNA at the P site activates the adjacent A site for aminoacyl‐tRNA binding.
UR - http://www.scopus.com/inward/record.url?scp=84987310102&partnerID=8YFLogxK
U2 - 10.1002/ijch.197200078
DO - 10.1002/ijch.197200078
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AN - SCOPUS:84987310102
SN - 0021-2148
VL - 10
SP - 775
EP - 785
JO - Israel Journal of Chemistry
JF - Israel Journal of Chemistry
IS - 4
ER -