Studies on the Specificity of the Interaction Between Peptidyl‐tRNA and E. Coli Ribosomes, Initiation and Elongation Factors

Several aspects of the binding of peptidyl‐tRNA to E. coli ribosomes were studied. The E. coli binding factor (EF₁) does not increase the rate of peptidyl‐tRNA binding. From the results obtained it was concluded that two molecules of Gly₂ Phe‐tRNA can bind to one and the same ribosome, but no new peptide bond was formed. Gly, Phe‐tRNA at the A site inhibited the puromycin reaction of Gly₂ Phe‐tRNA at the P site of the same ribosome. The binding of peptidyl‐tRNA at the P site activates the adjacent A site for aminoacyl‐tRNA binding.