TY - JOUR
T1 - Substitution of just five nucleotides at and around the transcription start site of rat β-actin promoter is sufficient to render the resulting transcript a subject for translational control
AU - Biberman, Yael
AU - Meyuhas, Oded
PY - 1997/4/1
Y1 - 1997/4/1
N2 - Vertebrate mRNAs with a 5' terminal oligopyrimidine tract (5' TOP), including those encoding ribosomal proteins and elongation factors, are candidates for translational control in a growth-dependent fashion. The present study was designed to determine the minimal cis-regulatory element involved in this mode of regulation. We selected rat p-actin mRNA, a typical translationally uncontrolled transcript, as a subject for gain-of-function analysis. Mutations at and around its cap site leading to the formation of a 7 pyrimidines long 5' TOP render the resulting transcript translationally repressed upon growth arrest of lymphosarcoma cells. In contrast, growth-dependent translational control of this mRNA in fibroblasts requires, in addition, a GC motif downstream of the 5' TOP, A similar motif is present in all ribosomal protein mRNAs shown to be translationally controlled.
AB - Vertebrate mRNAs with a 5' terminal oligopyrimidine tract (5' TOP), including those encoding ribosomal proteins and elongation factors, are candidates for translational control in a growth-dependent fashion. The present study was designed to determine the minimal cis-regulatory element involved in this mode of regulation. We selected rat p-actin mRNA, a typical translationally uncontrolled transcript, as a subject for gain-of-function analysis. Mutations at and around its cap site leading to the formation of a 7 pyrimidines long 5' TOP render the resulting transcript translationally repressed upon growth arrest of lymphosarcoma cells. In contrast, growth-dependent translational control of this mRNA in fibroblasts requires, in addition, a GC motif downstream of the 5' TOP, A similar motif is present in all ribosomal protein mRNAs shown to be translationally controlled.
KW - Oligopyrimidine tract
KW - Polysome
KW - Ribosomal protein mRNA
KW - Translational regulation
UR - http://www.scopus.com/inward/record.url?scp=0030929822&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)00234-2
DO - 10.1016/S0014-5793(97)00234-2
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C2 - 9108314
AN - SCOPUS:0030929822
SN - 0014-5793
VL - 405
SP - 333
EP - 336
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -