Substitution of just five nucleotides at and around the transcription start site of rat β-actin promoter is sufficient to render the resulting transcript a subject for translational control

Yael Biberman, Oded Meyuhas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Vertebrate mRNAs with a 5' terminal oligopyrimidine tract (5' TOP), including those encoding ribosomal proteins and elongation factors, are candidates for translational control in a growth-dependent fashion. The present study was designed to determine the minimal cis-regulatory element involved in this mode of regulation. We selected rat p-actin mRNA, a typical translationally uncontrolled transcript, as a subject for gain-of-function analysis. Mutations at and around its cap site leading to the formation of a 7 pyrimidines long 5' TOP render the resulting transcript translationally repressed upon growth arrest of lymphosarcoma cells. In contrast, growth-dependent translational control of this mRNA in fibroblasts requires, in addition, a GC motif downstream of the 5' TOP, A similar motif is present in all ribosomal protein mRNAs shown to be translationally controlled.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalFEBS Letters
Volume405
Issue number3
DOIs
StatePublished - 1 Apr 1997

Keywords

  • Oligopyrimidine tract
  • Polysome
  • Ribosomal protein mRNA
  • Translational regulation

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