Sucrose promotes stem branching through cytokinin

Bolaji Babajide Salam, Francois Barbier, Raz Danieli, Paula Teper-Bamnolker, Carmit Ziv, Lukáš Spíchal, Kalaivani Aruchamy, Yula Shnaider, Diana Leibman, Felix Shaya, Mira Carmeli-Weissberg, Amit Gal-On, Jiming Jiang, Naomi Ori, Christine Beveridge, Dani Eshel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.). However, the mechanism by which sugars affect shoot branching remains mostly unknown. In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions. Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength. These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose. CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing. Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.

Original languageAmerican English
Pages (from-to)1708-1721
Number of pages14
JournalPlant Physiology
Volume185
Issue number4
DOIs
StatePublished - Apr 2021

Bibliographical note

Publisher Copyright:
© American Society of Plant Biologists 2021.

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