TY - JOUR
T1 - Sucrose promotes stem branching through cytokinin
AU - Salam, Bolaji Babajide
AU - Barbier, Francois
AU - Danieli, Raz
AU - Teper-Bamnolker, Paula
AU - Ziv, Carmit
AU - Spíchal, Lukáš
AU - Aruchamy, Kalaivani
AU - Shnaider, Yula
AU - Leibman, Diana
AU - Shaya, Felix
AU - Carmeli-Weissberg, Mira
AU - Gal-On, Amit
AU - Jiang, Jiming
AU - Ori, Naomi
AU - Beveridge, Christine
AU - Eshel, Dani
N1 - Publisher Copyright:
© American Society of Plant Biologists 2021.
PY - 2021/4
Y1 - 2021/4
N2 - Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.). However, the mechanism by which sugars affect shoot branching remains mostly unknown. In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions. Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength. These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose. CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing. Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.
AB - Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.). However, the mechanism by which sugars affect shoot branching remains mostly unknown. In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions. Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength. These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose. CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing. Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.
UR - http://www.scopus.com/inward/record.url?scp=85105263745&partnerID=8YFLogxK
U2 - 10.1093/plphys/kiab003
DO - 10.1093/plphys/kiab003
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C2 - 33793932
AN - SCOPUS:85105263745
SN - 0032-0889
VL - 185
SP - 1708
EP - 1721
JO - Plant Physiology
JF - Plant Physiology
IS - 4
ER -