Sucrose-stimulated subsecond transient increase in cGMP level in rat intact circumvallate taste bud cells

Valery Krizhanovsky, Orly Agamy, Michael Naim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Initial sweet taste transduction is expected to occur in the subsecond time range. We demonstrate a rapid and transient (75-250 ms) increase of cGMP (but not cAMP) level in rat intact circumvallate taste cells after stimulation by sucrose. This rapid increase does not occur in nonsensory epithelial cells. Pre-treatment with a nonspecific phosphodiesterase (PDE) inhibitor (IBMX), a specific cAMP-PDE4 inhibitor (denbufylline), or an adenylyl cyclase activator (forskolin) all increased basal cAMP and abolished the sucrose-stimulated cGMP increase at 150 ms. Pretreatment with a soluble guanylyl cyclase inhibitor (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one) reduced, whereas a specific cGMP-PDE inhibitor (zaprinast) abolished, the sucrose-stimulated cGMP increase. It is proposed that cGMP is involved in the initial stage of sugar taste transduction and that cGMP is more significant than cAMP at this stage. Activation of soluble guanylyl cyclase and inhibition of cGMP-PDE may be involved in the transient elevation of cGMP in response to sucrose stimulation. Moreover, it appears that cAMP level must remain low for sucrose to stimulate an increase in cGMP.

Original languageEnglish
Pages (from-to)C120-C125
JournalAmerican Journal of Physiology - Cell Physiology
Volume279
Issue number1 48-1
DOIs
StatePublished - 2000

Keywords

  • Cyclic adenosine 5'- monophosphate
  • Cyclic guanosine 5'-monophosphate
  • Phosphodiesterase inhibitors
  • Sweet taste

Fingerprint

Dive into the research topics of 'Sucrose-stimulated subsecond transient increase in cGMP level in rat intact circumvallate taste bud cells'. Together they form a unique fingerprint.

Cite this