Sugar-dependent gibberellin-induced chalcone synthase gene expression in Petunia corollas

Delila Moalem-Beno, Guy Tamari, Yael Leitner-Dagan, Amihud Borochov, David Weiss*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

The induction of anthocyanin synthesis and anthocyanin biosynthetic gene expression in detached petunia (Petunia hybrida) corollas by gibberellic acid (GA3) requires sucrose. Neither sucrose nor GA3 alone can induce these processes. We found that GA3 enhances sucrose uptake by 20 to 30%, and we tested whether this is the mechanism by which the hormone induces gene expression. Changing the intracellular level of sucrose with the inhibitors p-chloromercuribenzenesulfonic acid and vanadate did not inhibit the induction of chalcone synthase gene (chs) expression by GA3. Growing detached corollas in various sucrose concentrations did not affect the induction of the gene but did affect its level of expression and the level of anthocyanin accumulated. Only metabolic sugars promoted GA3-induced anthocyanin accumulation. Mannitol and sorbitol had no effect and 3-O-methylglucose only slightly promoted chs expression and anthocyanin accumulation. Our results do not support the suggestion that sugars act as specific signals in the activation of anthocyanin biosynthetic gene expression during petunia corolla development. We suggest that sugars are essential as general sources of carbohydrates for carbon metabolism, upon which the induction of pigmentation is dependent.

Original languageAmerican English
Pages (from-to)419-424
Number of pages6
JournalPlant Physiology
Volume113
Issue number2
DOIs
StatePublished - Feb 1997

Fingerprint

Dive into the research topics of 'Sugar-dependent gibberellin-induced chalcone synthase gene expression in Petunia corollas'. Together they form a unique fingerprint.

Cite this