Abstract
Growth of Salinibacter ruber, a red, extremely halophilic bacterium phylogenetically affiliated with the Flavobacterium/Cytophaga branch of the domain Bacteria, is stimulated by a small number of sugars (glucose, maltose, starch at 1 g l-1). Glucose consumption starts after other substrates have been depleted. Glucose metabolism proceeds via a constitutive, salt-inhibited hexokinase and a constitutive salt-dependent nicotinamide adenine dinucleotide phosphate (NADP)-linked glucose-6-phosphate dehydrogenase. Glucose dehydrogenase and fructose-1,6-bisphosphate aldolase activity could not be detected. It is therefore suggested that Salinibacter metabolizes glucose by the classic Entner-Doudoroff pathway and not by the Embden-Meyerhof glycolytic pathway or by the modified Entner-Doudoroff pathway present in halophilic Archaea of the family Halobacteriaceae, in which the phosphorylation step is postponed. However, activity of 2-keto-3-deoxy-6-phosphogluconate aldolase could not be detected in extracts of Salinibacter cells, whether or not grown in the presence of glucose.
Original language | English |
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Pages (from-to) | 83-87 |
Number of pages | 5 |
Journal | FEMS Microbiology Letters |
Volume | 223 |
Issue number | 1 |
DOIs | |
State | Published - 6 Jun 2003 |
Bibliographical note
Funding Information:This study was supported by a grant from the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities.
Keywords
- 2-Keto-3-deoxy-6-phosphogluconate aldolase
- Entner-Doudoroff pathway
- Fructose-1,6-bisphosphate aldolase
- Glucose
- Glucose-6-phosphate dehydrogenase
- Hexokinase
- Salinibacter ruber