Sugars regulate sucrose transporter gene expression in citrus

Chun Yao Li; Jian Xin Shi; David Weiss; Eliezer E. Goldschmidt

doi:10.1016/S0006-291X(03)00978-1

Sugars regulate sucrose transporter gene expression in citrus

Chun Yao Li, Jian Xin Shi, David Weiss, Eliezer E. Goldschmidt

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We report the isolation and characterization of two sucrose transporter cDNAs (CitSUT1 and CitSUT2) from citrus. CitSUT1 and CitSUT2 encode putative proteins (CitSUT1 and CitSUT2) of 528 and 607 amino acids, respectively. CitSUT1 and CitSUT2 share high similarities with sucrose transporters isolated from other plants. The expression of CitSUT1 in mature leaf discs is repressed by exogenous sucrose, glucose, mannose, and the glucose analog 2-deoxyglucose but not by another glucose analog 3-O-methylglucose, indicating a hexokinase (HXK)-mediated signaling pathway. CitSUT2 expression is not affected by exogenous sugars. Whereas CitSUT1 expresses strongly in source, sugar exporting organs, CitSUT2 expresses more strongly in sink, sugar importing organs, suggesting different physiological roles for these sucrose transporters.

Original language	English
Pages (from-to)	402-407
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	306
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(03)00978-1
State	Published - 27 Jun 2003

Bibliographical note

Funding Information:
Thanks are due to Dr. D. Granot (The Agricultural Research Organization, Bet Dagan, Israel) for critical reading of the manuscript. The financial support by the Israel Citrus Marketing Board is gratefully acknowledged.

Keywords

Citrus
Glucose
Hexokinase
Source/sink
Sucrose
Sucrose transporter
Sugar response

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10.1016/S0006-291X(03)00978-1

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title = "Sugars regulate sucrose transporter gene expression in citrus",

abstract = "We report the isolation and characterization of two sucrose transporter cDNAs (CitSUT1 and CitSUT2) from citrus. CitSUT1 and CitSUT2 encode putative proteins (CitSUT1 and CitSUT2) of 528 and 607 amino acids, respectively. CitSUT1 and CitSUT2 share high similarities with sucrose transporters isolated from other plants. The expression of CitSUT1 in mature leaf discs is repressed by exogenous sucrose, glucose, mannose, and the glucose analog 2-deoxyglucose but not by another glucose analog 3-O-methylglucose, indicating a hexokinase (HXK)-mediated signaling pathway. CitSUT2 expression is not affected by exogenous sugars. Whereas CitSUT1 expresses strongly in source, sugar exporting organs, CitSUT2 expresses more strongly in sink, sugar importing organs, suggesting different physiological roles for these sucrose transporters.",

keywords = "Citrus, Glucose, Hexokinase, Source/sink, Sucrose, Sucrose transporter, Sugar response",

author = "Li, {Chun Yao} and Shi, {Jian Xin} and David Weiss and Goldschmidt, {Eliezer E.}",

note = "Funding Information: Thanks are due to Dr. D. Granot (The Agricultural Research Organization, Bet Dagan, Israel) for critical reading of the manuscript. The financial support by the Israel Citrus Marketing Board is gratefully acknowledged.",

year = "2003",

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doi = "10.1016/S0006-291X(03)00978-1",

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T1 - Sugars regulate sucrose transporter gene expression in citrus

AU - Li, Chun Yao

AU - Shi, Jian Xin

AU - Weiss, David

AU - Goldschmidt, Eliezer E.

N1 - Funding Information: Thanks are due to Dr. D. Granot (The Agricultural Research Organization, Bet Dagan, Israel) for critical reading of the manuscript. The financial support by the Israel Citrus Marketing Board is gratefully acknowledged.

PY - 2003/6/27

Y1 - 2003/6/27

N2 - We report the isolation and characterization of two sucrose transporter cDNAs (CitSUT1 and CitSUT2) from citrus. CitSUT1 and CitSUT2 encode putative proteins (CitSUT1 and CitSUT2) of 528 and 607 amino acids, respectively. CitSUT1 and CitSUT2 share high similarities with sucrose transporters isolated from other plants. The expression of CitSUT1 in mature leaf discs is repressed by exogenous sucrose, glucose, mannose, and the glucose analog 2-deoxyglucose but not by another glucose analog 3-O-methylglucose, indicating a hexokinase (HXK)-mediated signaling pathway. CitSUT2 expression is not affected by exogenous sugars. Whereas CitSUT1 expresses strongly in source, sugar exporting organs, CitSUT2 expresses more strongly in sink, sugar importing organs, suggesting different physiological roles for these sucrose transporters.

AB - We report the isolation and characterization of two sucrose transporter cDNAs (CitSUT1 and CitSUT2) from citrus. CitSUT1 and CitSUT2 encode putative proteins (CitSUT1 and CitSUT2) of 528 and 607 amino acids, respectively. CitSUT1 and CitSUT2 share high similarities with sucrose transporters isolated from other plants. The expression of CitSUT1 in mature leaf discs is repressed by exogenous sucrose, glucose, mannose, and the glucose analog 2-deoxyglucose but not by another glucose analog 3-O-methylglucose, indicating a hexokinase (HXK)-mediated signaling pathway. CitSUT2 expression is not affected by exogenous sugars. Whereas CitSUT1 expresses strongly in source, sugar exporting organs, CitSUT2 expresses more strongly in sink, sugar importing organs, suggesting different physiological roles for these sucrose transporters.

KW - Citrus

KW - Glucose

KW - Hexokinase

KW - Source/sink

KW - Sucrose

KW - Sucrose transporter

KW - Sugar response

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EP - 407

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Sugars regulate sucrose transporter gene expression in citrus

Abstract

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