Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

Israel Alshanski; Joanna Blaszkiewicz; Evgeniy Mervinetsky; Jörg Rademann; Shlomo Yitzchaik; Mattan Hurevich

doi:10.1002/chem.201901538

Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

Israel Alshanski, Joanna Blaszkiewicz, Evgeniy Mervinetsky, Jörg Rademann^*, Shlomo Yitzchaik, Mattan Hurevich

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Sulfated saccharides are an essential part of extracellular matrices, and they are involved in a large number of interactions. Sulfated saccharide matrices in organisms accumulate heavy metal ions in addition to other essential metal ions. Accumulation of heavy metal ions alters the function of the organisms and cells, resulting in severe and irreversible damage. The effect of the sulfation pattern of saccharides on heavy metal binding preferences is enigmatic because the accessibility to structurally defined sulfated saccharides is limited and because standard analytical techniques cannot be used to quantify these interactions. We developed a new strategy that combines enzymatic and chemical synthesis with surface chemistry and label-free electrochemical sensing to study the interactions between well-defined sulfated saccharides and heavy metal ions. By using these tools we showed that the sulfation pattern of hyaluronic acid governs their heavy metal ions binding preferences.

Original language	American English
Pages (from-to)	12083-12090
Number of pages	8
Journal	Chemistry - A European Journal
Volume	25
Issue number	52
DOIs	https://doi.org/10.1002/chem.201901538
State	Published - 18 Sep 2019

Bibliographical note

Funding Information:
S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis.

Funding Information:
S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis.

Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

analytical methods
carbohydrates
extracellular matrices
surface chemistry
trace analysis

Access to Document

10.1002/chem.201901538

Cite this

@article{571a09be339442898464e0ebb1cc5841,

title = "Sulfation Patterns of Saccharides and Heavy Metal Ion Binding",

abstract = "Sulfated saccharides are an essential part of extracellular matrices, and they are involved in a large number of interactions. Sulfated saccharide matrices in organisms accumulate heavy metal ions in addition to other essential metal ions. Accumulation of heavy metal ions alters the function of the organisms and cells, resulting in severe and irreversible damage. The effect of the sulfation pattern of saccharides on heavy metal binding preferences is enigmatic because the accessibility to structurally defined sulfated saccharides is limited and because standard analytical techniques cannot be used to quantify these interactions. We developed a new strategy that combines enzymatic and chemical synthesis with surface chemistry and label-free electrochemical sensing to study the interactions between well-defined sulfated saccharides and heavy metal ions. By using these tools we showed that the sulfation pattern of hyaluronic acid governs their heavy metal ions binding preferences.",

keywords = "analytical methods, carbohydrates, extracellular matrices, surface chemistry, trace analysis",

author = "Israel Alshanski and Joanna Blaszkiewicz and Evgeniy Mervinetsky and J{\"o}rg Rademann and Shlomo Yitzchaik and Mattan Hurevich",

note = "Funding Information: S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis. Funding Information: S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis. Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2019",

month = sep,

day = "18",

doi = "10.1002/chem.201901538",

language = "American English",

volume = "25",

pages = "12083--12090",

journal = "Chemistry - A European Journal",

issn = "0947-6539",

publisher = "Wiley-VCH Verlag",

number = "52",

}

TY - JOUR

T1 - Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

AU - Alshanski, Israel

AU - Blaszkiewicz, Joanna

AU - Mervinetsky, Evgeniy

AU - Rademann, Jörg

AU - Yitzchaik, Shlomo

AU - Hurevich, Mattan

N1 - Funding Information: S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis. Funding Information: S.Y. is the Binjamin H. Birstein Chair in Chemistry. The authors would like to thank RECORD-IT project. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 664786. J.R. and J.B. acknowledge support of this work by the Deutsche Forschungsgemeinschaft (DFG) by funding the collaborative research center SFB-TR67 (Functional biomaterials for controlling healing processes of bone and skin, project A8). The authors would like to thank Shahar Dery and Dr. Vitaly Gutkin for their help with the analysis. Publisher Copyright: © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2019/9/18

Y1 - 2019/9/18

N2 - Sulfated saccharides are an essential part of extracellular matrices, and they are involved in a large number of interactions. Sulfated saccharide matrices in organisms accumulate heavy metal ions in addition to other essential metal ions. Accumulation of heavy metal ions alters the function of the organisms and cells, resulting in severe and irreversible damage. The effect of the sulfation pattern of saccharides on heavy metal binding preferences is enigmatic because the accessibility to structurally defined sulfated saccharides is limited and because standard analytical techniques cannot be used to quantify these interactions. We developed a new strategy that combines enzymatic and chemical synthesis with surface chemistry and label-free electrochemical sensing to study the interactions between well-defined sulfated saccharides and heavy metal ions. By using these tools we showed that the sulfation pattern of hyaluronic acid governs their heavy metal ions binding preferences.

AB - Sulfated saccharides are an essential part of extracellular matrices, and they are involved in a large number of interactions. Sulfated saccharide matrices in organisms accumulate heavy metal ions in addition to other essential metal ions. Accumulation of heavy metal ions alters the function of the organisms and cells, resulting in severe and irreversible damage. The effect of the sulfation pattern of saccharides on heavy metal binding preferences is enigmatic because the accessibility to structurally defined sulfated saccharides is limited and because standard analytical techniques cannot be used to quantify these interactions. We developed a new strategy that combines enzymatic and chemical synthesis with surface chemistry and label-free electrochemical sensing to study the interactions between well-defined sulfated saccharides and heavy metal ions. By using these tools we showed that the sulfation pattern of hyaluronic acid governs their heavy metal ions binding preferences.

KW - analytical methods

KW - carbohydrates

KW - extracellular matrices

KW - surface chemistry

KW - trace analysis

UR - http://www.scopus.com/inward/record.url?scp=85072509095&partnerID=8YFLogxK

U2 - 10.1002/chem.201901538

DO - 10.1002/chem.201901538

M3 - Article

C2 - 31155776

AN - SCOPUS:85072509095

SN - 0947-6539

VL - 25

SP - 12083

EP - 12090

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

IS - 52

ER -

Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this