TY - JOUR
T1 - Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus
AU - Shahak, Yosepha
AU - Klughammer, Christof
AU - Schreiber, Ulrich
AU - Padan, Etana
AU - Herrman, Inge
AU - Hauska, Günter
PY - 1994/2
Y1 - 1994/2
N2 - The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc1-complex via the ubiquinone pool.
AB - The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc1-complex via the ubiquinone pool.
KW - anoxygenic photosynthesis
KW - Chlorobium limicola
KW - electron transport
KW - LED array spectrophotometer
KW - Oselllatoria limnetiea
KW - sulfide-quinone reductase (SQR)
UR - http://www.scopus.com/inward/record.url?scp=0027995856&partnerID=8YFLogxK
U2 - 10.1007/BF00029384
DO - 10.1007/BF00029384
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0027995856
SN - 0166-8595
VL - 39
SP - 175
EP - 181
JO - Photosynthesis Research
JF - Photosynthesis Research
IS - 2
ER -