Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus

Yosepha Shahak; Christof Klughammer; Ulrich Schreiber; Etana Padan; Inge Herrman; Günter Hauska

doi:10.1007/BF00029384

Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus

Yosepha Shahak^*
, Christof Klughammer
, Ulrich Schreiber
, Etana Padan
, Inge Herrman
, Günter Hauska

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for V_max of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for K_m of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc₁-complex via the ubiquinone pool.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	Photosynthesis Research
Volume	39
Issue number	2
DOIs	https://doi.org/10.1007/BF00029384
State	Published - Feb 1994

Keywords

Chlorobium limicola
LED array spectrophotometer
Oselllatoria limnetiea
anoxygenic photosynthesis
electron transport
sulfide-quinone reductase (SQR)

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10.1007/BF00029384

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title = "Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus",

abstract = "The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc1-complex via the ubiquinone pool.",

keywords = "Chlorobium limicola, LED array spectrophotometer, Oselllatoria limnetiea, anoxygenic photosynthesis, electron transport, sulfide-quinone reductase (SQR)",

author = "Yosepha Shahak and Christof Klughammer and Ulrich Schreiber and Etana Padan and Inge Herrman and G{\"u}nter Hauska",

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doi = "10.1007/BF00029384",

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T1 - Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus

AU - Shahak, Yosepha

AU - Klughammer, Christof

AU - Schreiber, Ulrich

AU - Padan, Etana

AU - Herrman, Inge

AU - Hauska, Günter

PY - 1994/2

Y1 - 1994/2

N2 - The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc1-complex via the ubiquinone pool.

AB - The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 μmoles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 μM were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc1-complex via the ubiquinone pool.

KW - Chlorobium limicola

KW - LED array spectrophotometer

KW - Oselllatoria limnetiea

KW - anoxygenic photosynthesis

KW - electron transport

KW - sulfide-quinone reductase (SQR)

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DO - 10.1007/BF00029384

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SN - 0166-8595

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SP - 175

EP - 181

JO - Photosynthesis Research

JF - Photosynthesis Research

IS - 2

ER -

Sulfide-quinone and sulfide-cytochrome reduction in Rhodobacter capsulatus

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Keywords

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