Abstract
B lymphocyte induced maturation protein-1 (Blimp-1) is a transcription repressor of the Krueppel-like family. Blimp-1 plays important roles in developmental processes, such as of germ cells and hair follicle stem cells. In B lymphocytes Blimp-1 orchestrates the terminal differentiation into plasma cells. We discovered that Blimp-1 undergoes SUMOylation by SUMO-1. This SUMOylation is modulated by the SUMO protease SENP1. While Blimp-1 is relatively stable in 293T cells, a fusion with SUMO1 rendered it to rapid proteasomal degradation. Increase in SENP1 activity stabilized Blimp-1, while a decrease promoted its degradation. Our data indicate that SUMOylation of Blimp-1 regulates its intracellular stability. Structured summary of protein interactions: Blimp1 physically interacts with SUMO1 by anti tag coimmunoprecipitation (View Interaction 1, 2). SUMO1 physically interacts with Blimp1 by anti tag coimmunoprecipitation (View interaction).
Original language | English |
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Pages (from-to) | 2405-2409 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 585 |
Issue number | 15 |
DOIs | |
State | Published - 4 Aug 2011 |
Bibliographical note
Funding Information:This work was supported by grants from the German Israel Foundation 1005/08 (BT and AW). The authors wish to declare no financial conflict of interests.
Keywords
- Plasma cell
- Proteasome
- SENP1
- SUMO protease