15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum

Jeremias C. Zill, Zhihui He, Marcus Tank, Bryan H. Ferlez, Daniel P. Canniffe, Yigal Lahav, Peter Bellstedt, A. Alia, Igor Schapiro, John H. Golbeck, Donald A. Bryant, Jörg Matysik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in the homodimeric, type-1 photochemical reaction centers (RCs) of the acidobacterium, Chloracidobacterium (Cab.) thermophilum, by 15N magic-angle spinning (MAS) solid-state NMR under continuous white-light illumination. Three light-induced emissive (negative) signals are detected. In the RCs of Cab. thermophilum, three types of (bacterio)chlorophylls have previously been identified: bacteriochlorophyll a (BChl a), chlorophyll a (Chl a), and Zn-bacteriochlorophyll a′ (Zn-BChl a′) (Tsukatani et al. in J Biol Chem 287:5720–5732, 2012). Based upon experimental and quantum chemical 15N NMR data, we assign the observed signals to a Chl a cofactor. We exclude Zn-BChl because of its measured spectroscopic properties. We conclude that Chl a is the primary electron acceptor, which implies that the primary donor is most likely Zn-BChl a′. Chl a and 81-OH Chl a have been shown to be the primary electron acceptors in green sulfur bacteria and heliobacteria, respectively, and thus a Chl a molecule serves this role in all known homodimeric type-1 RCs.

Original languageAmerican English
Pages (from-to)295-305
Number of pages11
JournalPhotosynthesis Research
Volume137
Issue number2
DOIs
StatePublished - 1 Aug 2018

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media B.V., part of Springer Nature.

Keywords

  • Chloracidobacterium thermophilum
  • Chlorophototrophy
  • N-MAS NMR
  • Photo-CIDNP
  • Reaction centers
  • Zn-BChl a′

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