Suppression of a Prm mutant by the sar mutation for the synthesis of repressor by bacteriophage lambda

Ariella Oppenheim*

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

4 Scopus citations

Abstract

The construction of a λprm116sar2a double mutant is described. The prm- mutation was characterized by the inability of the mutant bacteriophage to synthesize repressor from the promoter of maintenance of lysogeny, Prm (Gussin et al., 1975). The sar mutation, located near the origin of phage DNA replication, was shown to suppress cis-acting clear mutations (cy) for the synthesis of repressor during the establishment of lysogeny (Honigman et al., 1975). The present study shows that the inability to maintain lysogeny due to the prm- mutation is suppressed by the sar mutation. No repressor is synthesized when λprm infects immune cells whereas λprm sar2a synthesized as much as 30% of wild-type levels of repressor. The results indicate that transcription initiated near the origin of DNA replication may replace Prm for the synthesis of repressor.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalJournal of Molecular Biology
Volume111
Issue number1
DOIs
StatePublished - 25 Mar 1977

Fingerprint

Dive into the research topics of 'Suppression of a Prm mutant by the sar mutation for the synthesis of repressor by bacteriophage lambda'. Together they form a unique fingerprint.

Cite this