TY - JOUR
T1 - Suppression of a Prm mutant by the sar mutation for the synthesis of repressor by bacteriophage lambda
AU - Oppenheim, Ariella
PY - 1977/3/25
Y1 - 1977/3/25
N2 - The construction of a λprm116sar2a double mutant is described. The prm- mutation was characterized by the inability of the mutant bacteriophage to synthesize repressor from the promoter of maintenance of lysogeny, Prm (Gussin et al., 1975). The sar mutation, located near the origin of phage DNA replication, was shown to suppress cis-acting clear mutations (cy) for the synthesis of repressor during the establishment of lysogeny (Honigman et al., 1975). The present study shows that the inability to maintain lysogeny due to the prm- mutation is suppressed by the sar mutation. No repressor is synthesized when λprm infects immune cells whereas λprm sar2a synthesized as much as 30% of wild-type levels of repressor. The results indicate that transcription initiated near the origin of DNA replication may replace Prm for the synthesis of repressor.
AB - The construction of a λprm116sar2a double mutant is described. The prm- mutation was characterized by the inability of the mutant bacteriophage to synthesize repressor from the promoter of maintenance of lysogeny, Prm (Gussin et al., 1975). The sar mutation, located near the origin of phage DNA replication, was shown to suppress cis-acting clear mutations (cy) for the synthesis of repressor during the establishment of lysogeny (Honigman et al., 1975). The present study shows that the inability to maintain lysogeny due to the prm- mutation is suppressed by the sar mutation. No repressor is synthesized when λprm infects immune cells whereas λprm sar2a synthesized as much as 30% of wild-type levels of repressor. The results indicate that transcription initiated near the origin of DNA replication may replace Prm for the synthesis of repressor.
UR - http://www.scopus.com/inward/record.url?scp=0017623862&partnerID=8YFLogxK
U2 - 10.1016/S0022-2836(77)80134-4
DO - 10.1016/S0022-2836(77)80134-4
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.letter???
C2 - 853519
AN - SCOPUS:0017623862
SN - 0022-2836
VL - 111
SP - 83
EP - 89
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -