Suppression of allelic recombination and aneuploidy by cohesin is independent of CHK1 in saccharomyces cerevisiae

Shay Covo, Eric Chiou, Dmitry A. Gordenin, Michael A. Resnick

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Sister chromatid cohesion (SCC), which is established during DNA replication, ensures genome stability. Establishment of SCC is inhibited in G2. However, this inhibition is relived and SCC is established as a response to DNA damage, a process known as Damage Induced Cohesion (DIC). In yeast, Chk1, which is a kinase that functions in DNA damage signal transduction, is considered an activator of SCC through DIC. Nonetheless, here we show that, unlike SCC mutations, loss of CHK1 did not increase spontaneous or damage-induced allelic recombination or aneuploidy. We suggest that Chk1 has a redundant role in the control of DIC or that DIC is redundant for maintaining genome stability.

Original languageEnglish
Article numbere0113435
JournalPLoS ONE
Volume9
Issue number12
DOIs
StatePublished - 31 Dec 2014

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