TY - JOUR
T1 - Supramolecular cocaine-aptamer complexes activate biocatalytic cascades
AU - Freeman, Ronit
AU - Sharon, Etery
AU - Ran, Tel Vered
AU - Willner, Itamar
PY - 2009/4/15
Y1 - 2009/4/15
N2 - The anti-cocaine aptamer was fragmented into two nucleic acids, (1) and (2). The nucleic acid (1) was tethered at its 5′-end to aminoethyl nicotinamide adenine dinucleotide, amino-NAD+, or to horseradish peroxidase, HRP. The nucleic acid (2) was functionalized at its 3′-end with alcohol dehydrogenase, AlcDH, or with glucose oxidase, GOx. In the presence of cocaine, the supramolecular NAD+/AlcDH/cocaine-aptamer complex is formed, and the biocatalytic oxidation of ethanol is activated. Similarly, in the presence of cocaine, the GOx/HRP/cocaine-aptamer complex is formed, and this activates the biocatalytic cascade where glucose is oxidized by GOx to yield gluconic acid and H2O2, and the resulting hydrogen peroxide activates the HRP-biocatalyzed oxidation of 2,2′-azinobis-(3- ethylbenzthiazoline-6-sulfonate), ABTS2-. The systems may be considered as biomimetic prototypes for systems biology.
AB - The anti-cocaine aptamer was fragmented into two nucleic acids, (1) and (2). The nucleic acid (1) was tethered at its 5′-end to aminoethyl nicotinamide adenine dinucleotide, amino-NAD+, or to horseradish peroxidase, HRP. The nucleic acid (2) was functionalized at its 3′-end with alcohol dehydrogenase, AlcDH, or with glucose oxidase, GOx. In the presence of cocaine, the supramolecular NAD+/AlcDH/cocaine-aptamer complex is formed, and the biocatalytic oxidation of ethanol is activated. Similarly, in the presence of cocaine, the GOx/HRP/cocaine-aptamer complex is formed, and this activates the biocatalytic cascade where glucose is oxidized by GOx to yield gluconic acid and H2O2, and the resulting hydrogen peroxide activates the HRP-biocatalyzed oxidation of 2,2′-azinobis-(3- ethylbenzthiazoline-6-sulfonate), ABTS2-. The systems may be considered as biomimetic prototypes for systems biology.
UR - http://www.scopus.com/inward/record.url?scp=67849111859&partnerID=8YFLogxK
U2 - 10.1021/ja809496n
DO - 10.1021/ja809496n
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C2 - 19309141
AN - SCOPUS:67849111859
SN - 0002-7863
VL - 131
SP - 5028
EP - 5029
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 14
ER -