Supramolecular cocaine-aptamer complexes activate biocatalytic cascades

Ronit Freeman, Etery Sharon, Tel Vered Ran, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

101 Scopus citations

Abstract

The anti-cocaine aptamer was fragmented into two nucleic acids, (1) and (2). The nucleic acid (1) was tethered at its 5′-end to aminoethyl nicotinamide adenine dinucleotide, amino-NAD+, or to horseradish peroxidase, HRP. The nucleic acid (2) was functionalized at its 3′-end with alcohol dehydrogenase, AlcDH, or with glucose oxidase, GOx. In the presence of cocaine, the supramolecular NAD+/AlcDH/cocaine-aptamer complex is formed, and the biocatalytic oxidation of ethanol is activated. Similarly, in the presence of cocaine, the GOx/HRP/cocaine-aptamer complex is formed, and this activates the biocatalytic cascade where glucose is oxidized by GOx to yield gluconic acid and H2O2, and the resulting hydrogen peroxide activates the HRP-biocatalyzed oxidation of 2,2′-azinobis-(3- ethylbenzthiazoline-6-sulfonate), ABTS2-. The systems may be considered as biomimetic prototypes for systems biology.

Original languageEnglish
Pages (from-to)5028-5029
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number14
DOIs
StatePublished - 15 Apr 2009

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