Surface-Controlled Sialoside-Based Biosensing of Viral and Bacterial Neuraminidases

Israel Alshanski, Suraj Toraskar, Daniel Gordon-Levitan, Marco Massetti, Prashant Jain, Luigi Vaccaro, Raghavendra Kikkeri*, Mattan Hurevich*, Shlomo Yitzchaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Neuraminidases (NA) are sialic acid-cleaving enzymes that are used by both bacteria and viruses. These enzymes have sialoside structure-related binding and cleaving preferences. Differentiating between these enzymes requires using a large array of hard-to-access sialosides. In this work, we used electrochemical impedimetric biosensing to differentiate among several pathogene-related NAs. We used a limited set of sialosides and tailored the surface properties. Various sialosides were grafted on two different surfaces with unique properties. Electrografting on glassy carbon electrodes provided low-density sialoside-functionalized surfaces with a hydrophobic submonolayer. A two-step assembly on gold electrodes provided a denser sialoside layer on a negatively charged submonolayer. The synthesis of each sialoside required dozens of laborious steps. Utilizing the unique protein-electrode interaction modes resulted in richer biodata without increasing the synthetic load. These principles allowed for profiling NAs and determining the efficacy of various antiviral inhibitors.

Original languageAmerican English
Pages (from-to)7471-7478
Number of pages8
JournalLangmuir
Volume40
Issue number14
DOIs
StatePublished - 9 Apr 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society

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