TY - JOUR
T1 - Surface Markers on Human B and T Lymphocytes
T2 - VII. Rosette Formation Between Peripheral T Lymphocytes and Lymphoblastoid B‐Cell Lines
AU - JONDAL, M.
AU - KLEIN, E.
AU - YEFENOF, E.
PY - 1975/4
Y1 - 1975/4
N2 - Almost 100% of peripheral T lymphocytes are shown to have the capacity to form rosettes with human lymphoblastoid B‐cell lines, predominantly at 4°C and with lines having surface‐bound IgG. Blast‐transformed T cells retained this capacity and formed rosettes even at 37°C Unstimulated T cells bound less readily to B‐cell blasts, stimulated by pokeweed mitogen for 72 hr. Even though rosettes, formed at 4°C, were stable for several hours at 72°C, no T‐cell‐mediated cytotoxicity could be detected during overnight incubation. Extreme pH values and trypsinization decreased rosette formation, whereas neuraminidase treatment enhanced the reaction. Rosette formation was independent of bivalent cations and unimpaired in the presence of inhibitors (NaF. NaN3), undiluted human or fetal call sera, protein A, sonicated sheep erythrocyte membranes, and normal or heat‐aggregated human IgG. Anti‐Ig, anti β2‐microglobulin, or anti‐T cell sera did not influence rosette formation.
AB - Almost 100% of peripheral T lymphocytes are shown to have the capacity to form rosettes with human lymphoblastoid B‐cell lines, predominantly at 4°C and with lines having surface‐bound IgG. Blast‐transformed T cells retained this capacity and formed rosettes even at 37°C Unstimulated T cells bound less readily to B‐cell blasts, stimulated by pokeweed mitogen for 72 hr. Even though rosettes, formed at 4°C, were stable for several hours at 72°C, no T‐cell‐mediated cytotoxicity could be detected during overnight incubation. Extreme pH values and trypsinization decreased rosette formation, whereas neuraminidase treatment enhanced the reaction. Rosette formation was independent of bivalent cations and unimpaired in the presence of inhibitors (NaF. NaN3), undiluted human or fetal call sera, protein A, sonicated sheep erythrocyte membranes, and normal or heat‐aggregated human IgG. Anti‐Ig, anti β2‐microglobulin, or anti‐T cell sera did not influence rosette formation.
UR - http://www.scopus.com/inward/record.url?scp=0016771887&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3083.1975.tb02625.x
DO - 10.1111/j.1365-3083.1975.tb02625.x
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C2 - 1080288
AN - SCOPUS:0016771887
SN - 0300-9475
VL - 4
SP - 259
EP - 266
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
IS - 3
ER -