Surface morphology of cultured myeloma cells: Bleb formation in cell variants with defects in immunoglobulin production and secretion

R. Laskov, R. Baumal, A. Polliack

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The surface morphology of cultured mouse myeloma cells was studied by scanning and transmission electron microscopy. Cell surface blebs and microvilli were observed in approximately 30% of the cells of some myeloma lines. Some of these blebs had a row of small vesicles at their bases. These vesicles resembled the clasmacytic vacuoles previously described in lymphocytes. Pulse-chase experiments showed that immunoglobulin (Ig) did not pass through free blebs during its secretion into the culture medium. Bleb formation was not affected by the inhibition of Ig synthesis or secretion by cycloheximide and dinitrophenol, respectively. A cloned myeloma cell line which was not observed to have blebs produced and secreted the same amount of Ig as another cloned cell line characterized by multiple surface blebs. It was concluded that bleb formation is not correlated with Ig production or secretion.

Original languageEnglish
Pages (from-to)361-372
Number of pages12
JournalRES Journal of the Reticuloendothelial Society
Volume23
Issue number5
StatePublished - 1978

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