We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor
Bibliographical noteFunding Information:
We are grateful to Professor Ronen Rapaport, Professor Nathalie Q. Balabanand, and Dr. Adi Vaknin for their professional advices and generous help in sharing their laboratory facilities. We acknowledge partial financial support from Yissum Research and Development Company of the Hebrew University of Jerusalem.
- Fourier transform spectroscopy
- infrared spectroscopy
- surface plasmons