Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control

Alexander Zilbershtein; Amir Bein; Vladislav Lirtsman; Betty Schwartz; Michael Golosovsky; Dan Davidov

doi:10.1117/1.JBO.19.11.111608

Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control

Alexander Zilbershtein^*, Amir Bein, Vladislav Lirtsman, Betty Schwartz, Michael Golosovsky, Dan Davidov

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca²⁺ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor

Original language	English
Article number	111608
Journal	Journal of Biomedical Optics
Volume	19
Issue number	11
DOIs	https://doi.org/10.1117/1.JBO.19.11.111608
State	Published - Nov 2014

Bibliographical note

Funding Information:
We are grateful to Professor Ronen Rapaport, Professor Nathalie Q. Balabanand, and Dr. Adi Vaknin for their professional advices and generous help in sharing their laboratory facilities. We acknowledge partial financial support from Yissum Research and Development Company of the Hebrew University of Jerusalem.

Keywords

Fourier transform spectroscopy
cells
infrared spectroscopy
sensors
surface plasmons
waveguides

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10.1117/1.JBO.19.11.111608

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abstract = "We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor",

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AU - Bein, Amir

AU - Lirtsman, Vladislav

AU - Schwartz, Betty

AU - Golosovsky, Michael

AU - Davidov, Dan

N1 - Funding Information: We are grateful to Professor Ronen Rapaport, Professor Nathalie Q. Balabanand, and Dr. Adi Vaknin for their professional advices and generous help in sharing their laboratory facilities. We acknowledge partial financial support from Yissum Research and Development Company of the Hebrew University of Jerusalem.

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Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control

Abstract

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Keywords

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