Surface plasmon resonance characterization of photoswitchable antigen-antibody interactions

Evgeny Kaganer; Roman Pogreb; Dan Davidov; Itamar Willner

doi:10.1021/la981147v

Surface plasmon resonance characterization of photoswitchable antigen-antibody interactions

Evgeny Kaganer, Roman Pogreb, Dan Davidov, Itamar Willner

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-Ab dissociates from the protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates that the average thickness of the antibody layer is ca. 70 angstroms which corresponds to a surface coverage of ca. 4.4×10^-9 mol·cm^-2.

Original language	English
Pages (from-to)	3920-3923
Number of pages	4
Journal	Langmuir
Volume	15
Issue number	11
DOIs	https://doi.org/10.1021/la981147v
State	Published - 25 May 1999

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title = "Surface plasmon resonance characterization of photoswitchable antigen-antibody interactions",

abstract = "Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-Ab dissociates from the protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates that the average thickness of the antibody layer is ca. 70 angstroms which corresponds to a surface coverage of ca. 4.4×10-9 mol·cm-2.",

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AU - Kaganer, Evgeny

AU - Pogreb, Roman

AU - Davidov, Dan

AU - Willner, Itamar

PY - 1999/5/25

Y1 - 1999/5/25

N2 - Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-Ab dissociates from the protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates that the average thickness of the antibody layer is ca. 70 angstroms which corresponds to a surface coverage of ca. 4.4×10-9 mol·cm-2.

AB - Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-Ab dissociates from the protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates that the average thickness of the antibody layer is ca. 70 angstroms which corresponds to a surface coverage of ca. 4.4×10-9 mol·cm-2.

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Surface plasmon resonance characterization of photoswitchable antigen-antibody interactions

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