Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity

Moshe Shemesh; Doron Steinberg

doi:10.1016/j.mimet.2005.06.002

Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity

Moshe Shemesh, Doron Steinberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.

Original language	English
Pages (from-to)	411-415
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2005.06.002
State	Published - Mar 2006
Externally published	Yes

Keywords

Biofilm
Fructosyltransferase
Immobilized enzyme
Oral bacteria
Real-time activity
SPR

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10.1016/j.mimet.2005.06.002

Cite this

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title = "Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity",

abstract = "The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.",

keywords = "Biofilm, Fructosyltransferase, Immobilized enzyme, Oral bacteria, Real-time activity, SPR",

author = "Moshe Shemesh and Doron Steinberg",

year = "2006",

month = mar,

doi = "10.1016/j.mimet.2005.06.002",

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volume = "64",

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journal = "Journal of Microbiological Methods",

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AU - Shemesh, Moshe

AU - Steinberg, Doron

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AB - The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.

KW - Biofilm

KW - Fructosyltransferase

KW - Immobilized enzyme

KW - Oral bacteria

KW - Real-time activity

KW - SPR

UR - https://www.scopus.com/pages/publications/32244433754

U2 - 10.1016/j.mimet.2005.06.002

DO - 10.1016/j.mimet.2005.06.002

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C2 - 16098618

AN - SCOPUS:32244433754

SN - 0167-7012

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EP - 415

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity

Abstract

Keywords

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