Swim training induces distinct osseous gene expression patterns in anosteocytic and osteocytic teleost fish

Josephine T. Tauer, Tobias Thiele, Catherine Julien, Lior Ofer, Paul Zaslansky, Ron Shahar, Bettina M. Willie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The traditional understanding of bone mechanosensation implicates osteocytes, canaliculi, and the lacunocanalicular network in biomechanical adaptation. However, recent findings challenge this notion, as shown in advanced teleost fish where anosteocytic bone lacking osteocytes are nevertheless responsive to mechanical load. To investigate specific molecular mechanisms involved in bone mechanoadaptation in osteocytic and anosteocytic fish bone, we conducted a 5-min single swim-training experiment with zebrafish and ricefish, respectively. Through RNASeq analysis of fish spines, analyzed at various time points following swim training, we uncovered distinct gene expression patterns in osteocytic and anosteocytic fish bones. Notably, osteocytic fish bone exhibited an early response to mechanical load, contrasting to a delayed response observed in anosteocytic fish bones, both within 8 h following stimulation. We identified an increase in osteoblast differentiation in anosteocytic bone following training, while chordoblast activity was delayed. This temporal response suggests a time-dependent adaptation in anosteocytic bone, indicating the presence of intricate feedback networks within bone that lacks osteocytes.

Original languageEnglish
Article number117125
JournalBone
Volume185
DOIs
StatePublished - Aug 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Keywords

  • Anosteocytic bone
  • Bone
  • Mechanoadaptation
  • Osteocytes
  • Osteogenesis

Fingerprint

Dive into the research topics of 'Swim training induces distinct osseous gene expression patterns in anosteocytic and osteocytic teleost fish'. Together they form a unique fingerprint.

Cite this