Synaptogenesis and myopathy under acetylcholinesterase overexpression

Efrat Lev-Lehman, Tamah Evron, Ron Shraga Broide, Eran Meshorer, Ilana Ariel, Shlomo Seidman, Hermona Soreq*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Environmental, congenital, and acquired immunological insults perturbing neuromuscular junction (NMJ) activity may induce a variety of debilitating neuromuscular pathologies. However, the molecular elements linking NMJ dysfunction to long-term myopathies are unknown. Here, we report dramatically elevated levels of mRNA encoding C-Fos and the 'readthrough' (R) variant of acetylcholinesterase (AChE) in muscles of transgenic mice overexpressing synaptic (S) AChE in motoneurons and in control mice treated with the irreversible cholinesterase inhibitor diisopropylfluorophosphonate (DFP). Tongue muscles from DFP-treated and AChES transgenic mice displayed exaggerated neurite branching and disorganized, wasting fibers. Moreover, diaphragm muscles from both transgenic and DFP-treated mice exhibited NMJ proliferation. 2'-O-methyl-protected antisense oligonucleotides targeted to AChE mRNA suppressed feedback upregulation of AChE and ameliorated DFP- induced NMJ proliferation. Our findings demonstrate common transcriptional responses to cholinergic NMJ stress of diverse origin, and implicate deregulated AChE expression in excessive neurite outgrowth, uncontrolled synaptogenesis, and myopathology.

Original languageAmerican English
Pages (from-to)93-105
Number of pages13
JournalJournal of Molecular Neuroscience
Volume14
Issue number1-2
DOIs
StatePublished - 2000

Keywords

  • Acetylcholinesterase/antisense
  • Cholinesterase inhibitors
  • Muscle pathology
  • Neuromuscular junction
  • Physiological stress

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