TY - JOUR
T1 - Synchrotron small angle x-ray scattering quantitatively detects angstrom level changes in the average radius of taxol-stabilized microtubules decorated with the microtubule-associated-protein tau
AU - Choi, Myung Chul
AU - Raviv, Uri
AU - Li, Youli
AU - Miller, Herbert P.
AU - Needleman, Daniel J.
AU - Kim, Mahn Won
AU - Wilson, Leslie
AU - Feinstein, Stuart C.
AU - Safinya, Cyrus R.
PY - 2011
Y1 - 2011
N2 - With the emerging proteomics era the scientific community is beginning the daunting task of understanding the structures and functions of a large number of self-assembling proteins. Here, our study was concerned with the effect of the microtubule-associated-protein (MAP) tau on the assembled structure of taxol-stabilized microtubules. Significantly, the synchrotron small angle x-ray scattering (SAXS) technique is able to quantitatively detect angstrom level changes in the average diameter of the microtubules modeled as a simple hollow nanotube with a fixed wall thickness. We show that the electrostatic binding of MAP tau isoforms to taxol-stabilized MTs leads to a controlled increase in the average radius of microtubules with increasing coverage of tau on the MT surface. The increase in the average diameter results from an increase in the distribution of protofilament numbers in MTs upon binding of MAP tau.
AB - With the emerging proteomics era the scientific community is beginning the daunting task of understanding the structures and functions of a large number of self-assembling proteins. Here, our study was concerned with the effect of the microtubule-associated-protein (MAP) tau on the assembled structure of taxol-stabilized microtubules. Significantly, the synchrotron small angle x-ray scattering (SAXS) technique is able to quantitatively detect angstrom level changes in the average diameter of the microtubules modeled as a simple hollow nanotube with a fixed wall thickness. We show that the electrostatic binding of MAP tau isoforms to taxol-stabilized MTs leads to a controlled increase in the average radius of microtubules with increasing coverage of tau on the MT surface. The increase in the average diameter results from an increase in the distribution of protofilament numbers in MTs upon binding of MAP tau.
UR - http://www.scopus.com/inward/record.url?scp=79952711262&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/272/1/012001
DO - 10.1088/1742-6596/272/1/012001
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AN - SCOPUS:79952711262
SN - 1742-6588
VL - 272
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012001
ER -