TY - JOUR
T1 - Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress
T2 - A probe of interprotofilament interactions
AU - Needleman, Daniel J.
AU - Ojeda-Lopez, Miguel A.
AU - Raviv, Uri
AU - Ewert, Kai
AU - Jones, Jayna B.
AU - Miller, Herbert P.
AU - Wilson, Leslie
AU - Safinya, Cyrus R.
N1 - Funding Information:
We acknowledge insightful discussions with Bob McMeeking and Robijn Bruinsma. Supported by NIH GM-59288 and NS-13560, NSF DMR-0203755, CTS-0103516, and CTS-0404444. DOE supports SSRL. NSF-DMR-0080034 supports the UCSB MRL.
PY - 2004/11/5
Y1 - 2004/11/5
N2 - A synchrotron small angle x-ray diffraction (SAXRD) study of microtubule (MT), subjected to osmotic stress and depletion attraction due to added poly-(ethylene-oxide) (PEO) was discussed. It was found that the solutions of MT undergo two transitions. SAXRD of microtubules shows the transition to rectangular bundles with noncircular buckled cross sections, followed by hexagonally packed bundles, under increasing osmotic stress. The new technique probes the strength of interprotofilament bonds, by which the chemotherapy drug taxol and associated proteins stabilize microtubules.
AB - A synchrotron small angle x-ray diffraction (SAXRD) study of microtubule (MT), subjected to osmotic stress and depletion attraction due to added poly-(ethylene-oxide) (PEO) was discussed. It was found that the solutions of MT undergo two transitions. SAXRD of microtubules shows the transition to rectangular bundles with noncircular buckled cross sections, followed by hexagonally packed bundles, under increasing osmotic stress. The new technique probes the strength of interprotofilament bonds, by which the chemotherapy drug taxol and associated proteins stabilize microtubules.
UR - http://www.scopus.com/inward/record.url?scp=19744379853&partnerID=8YFLogxK
U2 - 10.1103/PhysRevLett.93.198104
DO - 10.1103/PhysRevLett.93.198104
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C2 - 15600887
AN - SCOPUS:19744379853
SN - 0031-9007
VL - 93
SP - 198104-1-198104-4
JO - Physical Review Letters
JF - Physical Review Letters
IS - 19
M1 - 198104
ER -