Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress: A probe of interprotofilament interactions

Daniel J. Needleman; Miguel A. Ojeda-Lopez; Uri Raviv; Kai Ewert; Jayna B. Jones; Herbert P. Miller; Leslie Wilson; Cyrus R. Safinya

doi:10.1103/PhysRevLett.93.198104

Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress: A probe of interprotofilament interactions

Daniel J. Needleman^*, Miguel A. Ojeda-Lopez, Uri Raviv, Kai Ewert, Jayna B. Jones, Herbert P. Miller, Leslie Wilson, Cyrus R. Safinya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

A synchrotron small angle x-ray diffraction (SAXRD) study of microtubule (MT), subjected to osmotic stress and depletion attraction due to added poly-(ethylene-oxide) (PEO) was discussed. It was found that the solutions of MT undergo two transitions. SAXRD of microtubules shows the transition to rectangular bundles with noncircular buckled cross sections, followed by hexagonally packed bundles, under increasing osmotic stress. The new technique probes the strength of interprotofilament bonds, by which the chemotherapy drug taxol and associated proteins stabilize microtubules.

Original language	American English
Article number	198104
Pages (from-to)	198104-1-198104-4
Journal	Physical Review Letters
Volume	93
Issue number	19
DOIs	https://doi.org/10.1103/PhysRevLett.93.198104
State	Published - 5 Nov 2004
Externally published	Yes

Bibliographical note

Funding Information:
We acknowledge insightful discussions with Bob McMeeking and Robijn Bruinsma. Supported by NIH GM-59288 and NS-13560, NSF DMR-0203755, CTS-0103516, and CTS-0404444. DOE supports SSRL. NSF-DMR-0080034 supports the UCSB MRL.

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Needleman, D. J., Ojeda-Lopez, M. A., Raviv, U., Ewert, K., Jones, J. B., Miller, H. P., Wilson, L., & Safinya, C. R. (2004). Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress: A probe of interprotofilament interactions. Physical Review Letters, 93(19), 198104-1-198104-4. Article 198104. https://doi.org/10.1103/PhysRevLett.93.198104

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title = "Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress: A probe of interprotofilament interactions",

abstract = "A synchrotron small angle x-ray diffraction (SAXRD) study of microtubule (MT), subjected to osmotic stress and depletion attraction due to added poly-(ethylene-oxide) (PEO) was discussed. It was found that the solutions of MT undergo two transitions. SAXRD of microtubules shows the transition to rectangular bundles with noncircular buckled cross sections, followed by hexagonally packed bundles, under increasing osmotic stress. The new technique probes the strength of interprotofilament bonds, by which the chemotherapy drug taxol and associated proteins stabilize microtubules.",

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note = "Funding Information: We acknowledge insightful discussions with Bob McMeeking and Robijn Bruinsma. Supported by NIH GM-59288 and NS-13560, NSF DMR-0203755, CTS-0103516, and CTS-0404444. DOE supports SSRL. NSF-DMR-0080034 supports the UCSB MRL.",

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doi = "10.1103/PhysRevLett.93.198104",

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AU - Ewert, Kai

AU - Jones, Jayna B.

AU - Miller, Herbert P.

AU - Wilson, Leslie

AU - Safinya, Cyrus R.

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AB - A synchrotron small angle x-ray diffraction (SAXRD) study of microtubule (MT), subjected to osmotic stress and depletion attraction due to added poly-(ethylene-oxide) (PEO) was discussed. It was found that the solutions of MT undergo two transitions. SAXRD of microtubules shows the transition to rectangular bundles with noncircular buckled cross sections, followed by hexagonally packed bundles, under increasing osmotic stress. The new technique probes the strength of interprotofilament bonds, by which the chemotherapy drug taxol and associated proteins stabilize microtubules.

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Synchrotron X-ray diffraction study of microtubules buckling and bundling under osmotic stress: A probe of interprotofilament interactions

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