Synergism between cytotoxic cells and immune serum in a syngeneic lymphoma: characterization of two types of cytotoxic cells

M. O. De Landazuri, E. Kedar, J. L. Fahey

Research output: Contribution to journalArticlepeer-review

Abstract

The immune response of W/Fu rats to the syngeneic Gross virus induced lymphoma (C58NT) D was notable for the absence of serum blocking activity and the presence of serum potentiation of cellular cytotoxicity (synergistic effect). Heat inactivated isoimmune serum specifically augmented the in vitro cell mediated cytotoxicity (CMC) of lymphoid cells immune to the syngeneic lymphoma. The characterization of the effector cells mediating CMC and the synergistic effect was approached in several ways: elimination of T cells by anti thymocyte serum and complement completely abrogated to the CMC acitivity while not impairing the synergistic capacity; specific depletion of cytotoxic (CMC) lymphoid cells on monolayers of target cells bearing the sensitizing antigens considerably diminished the CMC activity, but did not affect the synergistic activity; depletion of Ig (Fc) receptor bearing cells (non T cells) markedly reduced the synergistic effect, but did not interfere with CMC activity. These findings indicate that, in this system, two forms of cell mediated cytotoxicity to tumor associated antigens are expressions of different lymphoid cell populations: CMC is mediated by T cells, whereas the synergistic capacity is a non T cell function.

Original languageEnglish
Pages (from-to)3-14
Number of pages12
JournalBEHRING INST.MITT.
Volumeno. 56
StatePublished - 1975
Externally publishedYes

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