Syngeneic lysis of reticuloendotheliosis virus-transformed cell lines transfected with Marek's disease virus genes by virus-specific cytotoxic T cells

Zehava Uni, W. D. Pratt, M. M. Miller, P. H. O'Connell, K. A. Schat*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Cell-mediated immune responses against Marek's disease virus (MDV) antigens were examined using reticuloendotheliosis virus (REV)-transformed cell lines of two haplotypes (B19B19 and B13B13). These cell lines were stably transfected with cloned fragments of MDV DNA resulting in the expression of the MDV-specific phosphoprotein pp38. Effector cells were obtained from P2a (B19B19) and S13 (B13B13) chickens at 7 days post inoculation with REV, oncogenic or attenuated serotype 1 MDV (JM-16/O and JM-16/A, respectively), serotype 2 MDV (SB-1), or herpesvirus of turkeys (HVT). Transfection of MDV genes did not influence the expression of Class I major histocompatibility complex antigens. The optimal effector to target cell ratio was determined to be 100:1. REV-sensitized effector cells lysed REV cell lines and REV cell lines transfected with MDV DNA in a syngeneic fashion. Effector cells from chickens inoculated with JM-16/O, JM-16/A, SB-1 or HVT lysed only the syngeneic, transfected cell lines, but not the parent REV cell lines. The percentage specific release caused by the MDV-sensitized effector cells was low, but statistically significant.

Original languageAmerican English
Pages (from-to)57-69
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume44
Issue number1
DOIs
StatePublished - Dec 1994
Externally publishedYes

Bibliographical note

Funding Information:
Zehava Unl was the recipient of a Postdoctoral Fellowship Grant No FI-0130-90 from BARD, The United States-Israel Bmatlonal Agricultural Research and Development Fund The research was supported m part by Grant No IS 146088 from BARD and by the Cooperative State Research Service, US Department of Agriculture under Project No 92-37204-8044 The production ofmonoclonal antibody C6B 12 was supported by NIH Grant AI21736

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