TY - JOUR
T1 - Synthesis and characterization of near IR fluorescent albumin nanoparticles for optical detection of colon cancer
AU - Cohen, Sarit
AU - Pellach, Michal
AU - Kam, Yossi
AU - Grinberg, Igor
AU - Corem-Salkmon, Enav
AU - Rubinstein, Abraham
AU - Margel, Shlomo
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Near IR (NIR) fluorescent human serum albumin (HSA) nanoparticles hold great promise as contrast agents for tumor diagnosis. HSA nanoparticles are considered to be biocompatible, non-toxic and non-immunogenic. In addition, NIR fluorescence properties of these nanoparticles are important for in vivo tumor diagnostics, with low autofluorescence and relatively deep penetration of NIR irradiation due to low absorption of biomatrices. The present study describes the synthesis of new NIR fluorescent HSA nanoparticles, by entrapment of a NIR fluorescent dye within the HSA nanoparticles, which also significantly increases the photostability of the dye. Tumor-targeting ligands such as peanut agglutinin (PNA) and anti-carcinoembryonic antigen antibodies (anti-CEA) were covalently conjugated to the NIR fluorescent albumin nanoparticles, increasing the potential fluorescent signal in tumors with upregulated corresponding receptors. Specific colon tumor detection by the NIR fluorescent HSA nanoparticles was demonstrated in a chicken embryo model and a rat model. In future work we also plan to encapsulate cancer drugs such as doxorubicin within the NIR fluorescent HSA nanoparticles for both colon cancer imaging and therapy.
AB - Near IR (NIR) fluorescent human serum albumin (HSA) nanoparticles hold great promise as contrast agents for tumor diagnosis. HSA nanoparticles are considered to be biocompatible, non-toxic and non-immunogenic. In addition, NIR fluorescence properties of these nanoparticles are important for in vivo tumor diagnostics, with low autofluorescence and relatively deep penetration of NIR irradiation due to low absorption of biomatrices. The present study describes the synthesis of new NIR fluorescent HSA nanoparticles, by entrapment of a NIR fluorescent dye within the HSA nanoparticles, which also significantly increases the photostability of the dye. Tumor-targeting ligands such as peanut agglutinin (PNA) and anti-carcinoembryonic antigen antibodies (anti-CEA) were covalently conjugated to the NIR fluorescent albumin nanoparticles, increasing the potential fluorescent signal in tumors with upregulated corresponding receptors. Specific colon tumor detection by the NIR fluorescent HSA nanoparticles was demonstrated in a chicken embryo model and a rat model. In future work we also plan to encapsulate cancer drugs such as doxorubicin within the NIR fluorescent HSA nanoparticles for both colon cancer imaging and therapy.
KW - Fluorescent nanoparticles
KW - HSA nanoparticles
KW - Keywords
KW - NIR fluorescence
KW - NIR fluorescent albumin nanoparticles
KW - Optical imaging
UR - http://www.scopus.com/inward/record.url?scp=84871370797&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2012.11.022
DO - 10.1016/j.msec.2012.11.022
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C2 - 25427507
AN - SCOPUS:84871370797
SN - 0928-4931
VL - 33
SP - 923
EP - 931
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 2
ER -