TY - JOUR
T1 - Synthesis, assembly and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells
AU - Laskov, Reuven
AU - Lanzerotti, Richard
AU - Scharff, Matthew D.
PY - 1971/3/14
Y1 - 1971/3/14
N2 - The kinetics, pathway, and efficiency of covalent assembly of mouse IgG2b immunoglobulin has been investigated using both tumor and cultured cells from the MPC 11 mouse myeloma. More than half of the interchain disulfide bonds were formed within three to five minutes after the synthesis and release of newly synthesized heavy and light chains and assembly was largely completed within 10 to 20 minutes after synthesis. Multiple pathways of assembly were used. Cells derived from the tumor are blocked in the covalent assembly of half molecules into IgG, but carry out the non-covalent assembly of half molecules to a higher polymer. This block in assembly may be due to the presence of a large excess of light chains rather than to a structural defect in the polypeptide chains.
AB - The kinetics, pathway, and efficiency of covalent assembly of mouse IgG2b immunoglobulin has been investigated using both tumor and cultured cells from the MPC 11 mouse myeloma. More than half of the interchain disulfide bonds were formed within three to five minutes after the synthesis and release of newly synthesized heavy and light chains and assembly was largely completed within 10 to 20 minutes after synthesis. Multiple pathways of assembly were used. Cells derived from the tumor are blocked in the covalent assembly of half molecules into IgG, but carry out the non-covalent assembly of half molecules to a higher polymer. This block in assembly may be due to the presence of a large excess of light chains rather than to a structural defect in the polypeptide chains.
UR - http://www.scopus.com/inward/record.url?scp=0015222704&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(71)90468-2
DO - 10.1016/0022-2836(71)90468-2
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C2 - 5102530
AN - SCOPUS:0015222704
SN - 0022-2836
VL - 56
SP - 327
EP - 339
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -