TY - JOUR
T1 - Synthesis of trinitrophenylaminolauric acid and the use of its glyceryl esters for assaying lipase by a spectrophotometric procedure
AU - Goldberg, R.
AU - Barenholz, Y.
AU - Gatt, S.
PY - 1978/11/22
Y1 - 1978/11/22
N2 - Trinitrophenylaminolauric acid was synthesized from ω-aminolauric acid and trinitrobenzenesulfonic acid and then condensed with glycerol to yield mono-, di- and triacylglyceryl esters of this acid. Hydrolysis of these glycerides was followed by isolation of the yellow fatty acid with the aid of one solvent extraction step and estimating its content by spectrophotometry. This procedure was used to assay the activities of lipases from hog pancreas, rat bile, microsomes of rat brain and Rhizopus arrhizus delamar.
AB - Trinitrophenylaminolauric acid was synthesized from ω-aminolauric acid and trinitrobenzenesulfonic acid and then condensed with glycerol to yield mono-, di- and triacylglyceryl esters of this acid. Hydrolysis of these glycerides was followed by isolation of the yellow fatty acid with the aid of one solvent extraction step and estimating its content by spectrophotometry. This procedure was used to assay the activities of lipases from hog pancreas, rat bile, microsomes of rat brain and Rhizopus arrhizus delamar.
UR - http://www.scopus.com/inward/record.url?scp=0018121283&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(78)90148-0
DO - 10.1016/0005-2760(78)90148-0
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C2 - 718973
AN - SCOPUS:0018121283
SN - 0005-2760
VL - 531
SP - 237
EP - 241
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 2
ER -