Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly

Wu Cheng Shen, Sukesh R. Bhaumik, Helen C. Causton, Itamar Simon, Xiaochun Zhu, Ezra G. Jennings, Tseng Hsing Wang, Richard A. Young, Michael R. Green*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

The general transcription factor TFIID is composed of the TATA box binding protein (TBP) and a set of conserved TBP-associated factors (TAFs). Here we report the completion of genome-wide expression profiling analyses of yeast strains bearing temperature-sensitive mutations in each of the 13 essential TAFs. The percentage of the yeast genome dependent on each TAF ranges from 3% (TAF2) to 59-61% (TAF9). Approximately 84% of yeast genes are dependent upon one or more TAFs and 16% of yeast genes are TAF independent. In addition, this complete analysis defines three distinct classes of yeast promoters whose transcriptional requirements for TAFs differ substantially. Using this collection of temperature-sensitive mutants, we show that in all cases the transcriptional dependence for a TAF can be explained by a requirement for TBP recruitment and assembly of the preinitiation complex (PIC). Unexpectedly, these assembly experiments reveal that TAF11 and TAF13 appear to provide the critical functional contacts with TBP during PIC assembly. Collectively, our results confirm and extend the proposal that individual TAFs have selective transcriptional roles and distinct functions.

Original languageAmerican English
Pages (from-to)3395-3402
Number of pages8
JournalEMBO Journal
Volume22
Issue number13
DOIs
StatePublished - 1 Jul 2003

Keywords

  • Chromatin immunoprecipitation
  • Genome-wide transcription
  • Preinitiation complex assembly
  • TAF
  • TBP

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