TY - JOUR
T1 - Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery
AU - Kafri, Moshe
AU - Patena, Weronika
AU - Martin, Lance
AU - Wang, Lianyong
AU - Gomer, Gillian
AU - Ergun, Sabrina L.
AU - Sirkejyan, Arthur K.
AU - Goh, Audrey
AU - Wilson, Alexandra T.
AU - Gavrilenko, Sophia E.
AU - Breker, Michal
AU - Roichman, Asael
AU - McWhite, Claire D.
AU - Rabinowitz, Joshua D.
AU - Cross, Frederick R.
AU - Wühr, Martin
AU - Jonikas, Martin C.
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023/12/7
Y1 - 2023/12/7
N2 - Photosynthesis is central to food production and the Earth's biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii, we identify with high confidence (false discovery rate [FDR] < 0.11) 70 poorly characterized genes required for photosynthesis. We then enable the functional characterization of these genes by providing a resource of proteomes of mutant strains, each lacking one of these genes. The data allow assignment of 34 genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Further analysis uncovers biogenesis/regulatory roles for at least seven proteins, including five photosystem I mRNA maturation factors, the chloroplast translation factor MTF1, and the master regulator PMR1, which regulates chloroplast genes via nuclear-expressed factors. Our work provides a rich resource identifying regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.
AB - Photosynthesis is central to food production and the Earth's biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii, we identify with high confidence (false discovery rate [FDR] < 0.11) 70 poorly characterized genes required for photosynthesis. We then enable the functional characterization of these genes by providing a resource of proteomes of mutant strains, each lacking one of these genes. The data allow assignment of 34 genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Further analysis uncovers biogenesis/regulatory roles for at least seven proteins, including five photosystem I mRNA maturation factors, the chloroplast translation factor MTF1, and the master regulator PMR1, which regulates chloroplast genes via nuclear-expressed factors. Our work provides a rich resource identifying regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.
KW - RNA sequencing
KW - genetics
KW - methionyl-tRNA formyltransferase
KW - photosynthesis
KW - photosystem I maturation factors
KW - protein localization
KW - protein profiling
KW - retrograde regulation
UR - http://www.scopus.com/inward/record.url?scp=85178149852&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2023.11.007
DO - 10.1016/j.cell.2023.11.007
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C2 - 38065083
AN - SCOPUS:85178149852
SN - 0092-8674
VL - 186
SP - 5638-5655.e25
JO - Cell
JF - Cell
IS - 25
ER -