T-cell hybridoma bearing heavy chain variable region determinants producing (T,G)-A--L-specific helper factor

Zelig Eshhar*, Ron N. Apte, Ilana Löwy, Yinon Ben-neriah, David Givol, Edna Mozes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Thymus-derived lymphocytes (T cells) exert their regulatory effect (help or suppression) on the antibody production by B cells either by direct cell to cell interaction or by soluble mediators or factors1,2. The low frequency of specific T cells, the heterogeneity of their responses and their relatively short life span have hampered the molecular characterization of the antigen recognition unit of T cells, and its structure is largely unknown. The lymphocyte hybridization technique, which has been found very useful for the production of B-cell hybridomas secreting specific monoclonal antibodies3, has also been used for the generation of homogeneous and stable T-cell hybridomas with unlimited growth potential4,5. So far the only specific effector function demonstrated in the established T hybridomas is the property to generate a factor(s) which suppresses antibody responses6-8. We now describe the establishment of hybrid lines which exhibit characteristic T-cell markers. One of the hybridomas (denoted R-9) releases into the culture supernatant factor(s) with helper activity specific to the synthetic polypeptide (T,G)-A--L and bears surface determinants of the immunoglobulin heavy chain variable region (VH). Such hybrid cell lines are of great value for studies on the nature of the T-cell receptor.

Original languageAmerican English
Pages (from-to)270-272
Number of pages3
JournalNature
Volume286
Issue number5770
DOIs
StatePublished - 1980
Externally publishedYes

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