Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Ido Amit; Liat Yakir; Menachem Katz; Yaara Zwang; Mina D. Marmor; Ami Citri; Keren Shtiegman; Iris Alroy; Shmuel Tuvia; Yuval Reiss; Eli Roubini; Maya Cohen; Ron Wides; Eran Bacharach; Ullrich Schubert; Yosef Yarden

doi:10.1101/gad.294904

Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Ido Amit, Liat Yakir, Menachem Katz, Yaara Zwang, Mina D. Marmor, Ami Citri, Keren Shtiegman, Iris Alroy, Shmuel Tuvia, Yuval Reiss, Eli Roubini, Maya Cohen, Ron Wides, Eran Bacharach, Ullrich Schubert, Yosef Yarden^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

Original language	English
Pages (from-to)	1737-1752
Number of pages	16
Journal	Genes and Development
Volume	18
Issue number	14
DOIs	https://doi.org/10.1101/gad.294904
State	Published - 15 Jul 2004
Externally published	Yes

Keywords

Endocytosis
Growth factor
HIV
Signal transduction
Ubiquitin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Amit, I., Yakir, L., Katz, M., Zwang, Y., Marmor, M. D., Citri, A., Shtiegman, K., Alroy, I., Tuvia, S., Reiss, Y., Roubini, E., Cohen, M., Wides, R., Bacharach, E., Schubert, U., & Yarden, Y. (2004). Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes and Development, 18(14), 1737-1752. https://doi.org/10.1101/gad.294904

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abstract = "The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.",

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Amit, I, Yakir, L, Katz, M, Zwang, Y, Marmor, MD, Citri, A, Shtiegman, K, Alroy, I, Tuvia, S, Reiss, Y, Roubini, E, Cohen, M, Wides, R, Bacharach, E, Schubert, U & Yarden, Y 2004, 'Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding', Genes and Development, vol. 18, no. 14, pp. 1737-1752. https://doi.org/10.1101/gad.294904

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AU - Yakir, Liat

AU - Katz, Menachem

AU - Zwang, Yaara

AU - Marmor, Mina D.

AU - Citri, Ami

AU - Shtiegman, Keren

AU - Alroy, Iris

AU - Tuvia, Shmuel

AU - Reiss, Yuval

AU - Roubini, Eli

AU - Cohen, Maya

AU - Wides, Ron

AU - Bacharach, Eran

AU - Schubert, Ullrich

AU - Yarden, Yosef

PY - 2004/7/15

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AB - The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

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KW - Growth factor

KW - HIV

KW - Signal transduction

KW - Ubiquitin

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Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Abstract

Keywords

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