Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Ido Amit; Liat Yakir; Menachem Katz; Yaara Zwang; Mina D. Marmor; Ami Citri; Keren Shtiegman; Iris Alroy; Shmuel Tuvia; Yuval Reiss; Eli Roubini; Maya Cohen; Ron Wides; Eran Bacharach; Ullrich Schubert; Yosef Yarden

doi:10.1101/gad.294904

Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Ido Amit
, Liat Yakir
, Menachem Katz
, Yaara Zwang
, Mina D. Marmor
, Ami Citri
, Keren Shtiegman
, Iris Alroy
, Shmuel Tuvia
, Yuval Reiss
, Eli Roubini
, Maya Cohen
, Ron Wides
, Eran Bacharach
, Ullrich Schubert
, Yosef Yarden^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

Original language	English
Pages (from-to)	1737-1752
Number of pages	16
Journal	Genes and Development
Volume	18
Issue number	14
DOIs	https://doi.org/10.1101/gad.294904
State	Published - 15 Jul 2004
Externally published	Yes

Keywords

Endocytosis
Growth factor
HIV
Signal transduction
Ubiquitin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1101/gad.294904

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Amit, I., Yakir, L., Katz, M., Zwang, Y., Marmor, M. D., Citri, A., Shtiegman, K., Alroy, I., Tuvia, S., Reiss, Y., Roubini, E., Cohen, M., Wides, R., Bacharach, E., Schubert, U., & Yarden, Y. (2004). Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes and Development, 18(14), 1737-1752. https://doi.org/10.1101/gad.294904

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abstract = "The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.",

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Amit, I, Yakir, L, Katz, M, Zwang, Y, Marmor, MD, Citri, A, Shtiegman, K, Alroy, I, Tuvia, S, Reiss, Y, Roubini, E, Cohen, M, Wides, R, Bacharach, E, Schubert, U & Yarden, Y 2004, 'Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding', Genes and Development, vol. 18, no. 14, pp. 1737-1752. https://doi.org/10.1101/gad.294904

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T1 - Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

AU - Amit, Ido

AU - Yakir, Liat

AU - Katz, Menachem

AU - Zwang, Yaara

AU - Marmor, Mina D.

AU - Citri, Ami

AU - Shtiegman, Keren

AU - Alroy, Iris

AU - Tuvia, Shmuel

AU - Reiss, Yuval

AU - Roubini, Eli

AU - Cohen, Maya

AU - Wides, Ron

AU - Bacharach, Eran

AU - Schubert, Ullrich

AU - Yarden, Yosef

PY - 2004/7/15

Y1 - 2004/7/15

N2 - The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

AB - The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

KW - Endocytosis

KW - Growth factor

KW - HIV

KW - Signal transduction

KW - Ubiquitin

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DO - 10.1101/gad.294904

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C2 - 15256501

AN - SCOPUS:3142742326

SN - 0890-9369

VL - 18

SP - 1737

EP - 1752

JO - Genes and Development

JF - Genes and Development

IS - 14

ER -

Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

Abstract

Keywords

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