Tal1 transgenic expression reveals absence of B lymphocytes

Alexey Palamarchuk, Nicola Zanesi, Rami I. Aqeilan, Alexey Efanov, Vadim Maximov, Urmila Santanam, John P. Hagan, Carlo M. Croce, Yuri Pekarsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


TAL1 oncogene encodes a helix-loop-helix transcription factor, Tal1, which is required for blood cell development, and its activation is a frequent event in T-cell acute lymphoblastic leukemia. Tal1 interacts and inhibits other helix-loop-helix factors such as E47 and HEB. To investigate the function of Tal1 in B cells, we generated Eμ-TAL1 transgenic mouse line, expressing Tal1 in mouse B-cell lineage. Fluorescence-activated cell sorting (FACS) analysis of lymphocytes isolated from spleens of five out of five founders reveals complete absence of IgM- or CD19-expressing cells. Only 2% to 3% of these cells were B220+ and 100% of B220+ cells were CD43+, indicating that these mice were able to make pro-B cells. Similarly, FACS analysis of bone marrow cells in Eμ-TAL1 mice revealed complete absence of B220+IgM+ and B220+CD19+ cells. Analysis of the recombination status of IgH genes revealed the presence of D-J but absence or drastic reduction of V-D-J rearrangements. Our results suggest that Tall overexpression in B cells results in a phenotype similar to that of B cells of E47/E2A knockout animals. This represents first in vivo evidence that Tal1 can completely inhibit E47/E2A function.

Original languageAmerican English
Pages (from-to)6014-6017
Number of pages4
JournalCancer Research
Issue number12
StatePublished - 15 Jun 2006
Externally publishedYes


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