Targeted in situ cross-linking mass spectrometry and integrative modeling reveal the architectures of three proteins from SARS-CoV-2

Moriya Slavin, Joanna Zamel, Keren Zohar, Tsiona Eliyahu, Merav Braitbard, Esther Brielle, Leah Baraz, Miri Stolovich-Rain, Ahuva Friedman, Dana G. Wolf, Alexander Rouvinski, Michal Linial*, Dina Schneidman-Duhovny, Nir Kalisman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Atomic structures of several proteins from the coronavirus family are still partial or unavailable. A possible reason for this gap is the instability of these proteins outside of the cellular context, thereby prompting the use of in-cell approaches. In situ cross-linking and mass spectrometry (in situ CLMS) can provide information on the structures of such proteins as they occur in the intact cell. Here, we applied targeted in situ CLMS to structurally probe Nsp1, Nsp2, and nucleocapsid (N) proteins from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and obtained cross-link sets with an average density of one cross-link per 20 residues. We then employed integrative modeling that computationally combined the cross-linking data with domain structures to determine full-length atomic models. For the Nsp2, the cross-links report on a complex topology with long-range interactions. Integrative modeling with structural prediction of individual domains by the AlphaFold2 system allowed us to generate a single consistent all-atom model of the full-length Nsp2. The model reveals three putative metal binding sites and suggests a role for Nsp2 in zinc regulation within the replication-transcription complex. For the N protein, we identified multiple intra- and interdomain cross-links. Our integrative model of the N dimer demonstrates that it can accommodate three single RNA strands simultaneously, both stereochemically and electrostatically. For the Nsp1, cross-links with the 40S ribosome were highly consistent with recent cryogenic electron microscopy structures. These results highlight the importance of cellular context for the structural probing of recalcitrant proteins and demonstrate the effectiveness of targeted in situ CLMS and integrative modeling.

Original languageAmerican English
Article numbere2103554118
JournalProceedings of the National Academy of Sciences of the United States of America
Volume118
Issue number34
DOIs
StatePublished - 24 Aug 2021

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. This work was supported by the Israel Science Foundation (ISF) 1768/15 (J.Z. and N.K.), ISF 3753/20 (K.Z., T.E., and M.L.), and ISF 1466/18; Israeli Ministry of Science and Technology (M.B. and D.S.-D.); and FSHD Global grant 41 (M.S.).

Funding Information:
This work was supported by the Israel Science Foundation (ISF) 1768/15 (J.Z. and N.K.), ISF 3753/20 (K.Z., T.E., and M.L.), and ISF 1466/18; Israeli Ministry of Science and Technology (M.B. and D.S.-D.); and FSHD Global grant 41 (M.S.).

Publisher Copyright:
© 2021 National Academy of Sciences. All rights reserved.

Keywords

  • In-cell techniques
  • Integrative modeling
  • Mass spectrometry
  • Structural biology
  • integrative modeling
  • mass spectrometry
  • structural biology
  • in-cell techniques

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