Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots

Gil Aizik, Nir Waiskopf, Majd Agbaria, Meital Ben-David-Naim, Mirjam M. Nordling-David, Doaa Jbara-Agbaria, Uri Banin, Gershon Golomb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Quantum dots offer superior optical features and hold a great potential as an imaging tool in comparison to ‘conventional’ fluorescent dyes. However, in vivo application in inflammatory-associated disorders is limited due to potential toxicity following systemic administration. Vascular inflammation contributes to cardiovascular diseases such as restenosis (re-narrowing of the artery following angioplasty), and poor prognosis is associated with the increased number of monocytes-derived macrophages (MDMs) in the arterial wall. Local administration of a suitable delivery system targeting MDMs could provide effective fluorescent imaging while minimizing systemic exposure and toxicity. We report here on the physicochemical characteristics and the structural stability of MDMs-targeted liposomal QDs (LipQDs), cellular uptake and cytotoxicity, the systemic biodistribution of LipQDs following local intra-luminal administration of LipQDs in carotid-injured rats vs. systemic administration, and imaging of QDs in the arterial tissue. The local treatment with LipQDs was found to be a suitable approach for targeting QDs to MDMs in the injured artery. In contrast to free QDs, the LipQDs formulation exhibited unique properties including structural and fluorescent stability, increased accumulation and retention for up to 24 h, and targeting properties enabling imaging of MDMs. MDMs imaging by targeted nanoparticles (NPs) could potentially serve for the detection of MDMs density in the injured artery for diagnostic purposes.

Original languageAmerican English
Pages (from-to)145-157
Number of pages13
JournalJournal of Controlled Release
Volume318
DOIs
StatePublished - Feb 2020

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

  • Fluorescent imaging
  • Liposomes
  • Local administration
  • Monocytes-derived macrophages
  • Quantum dots
  • Vascular injury

Fingerprint

Dive into the research topics of 'Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots'. Together they form a unique fingerprint.

Cite this