TY - JOUR
T1 - Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots
AU - Aizik, Gil
AU - Waiskopf, Nir
AU - Agbaria, Majd
AU - Ben-David-Naim, Meital
AU - Nordling-David, Mirjam M.
AU - Jbara-Agbaria, Doaa
AU - Banin, Uri
AU - Golomb, Gershon
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/2
Y1 - 2020/2
N2 - Quantum dots offer superior optical features and hold a great potential as an imaging tool in comparison to ‘conventional’ fluorescent dyes. However, in vivo application in inflammatory-associated disorders is limited due to potential toxicity following systemic administration. Vascular inflammation contributes to cardiovascular diseases such as restenosis (re-narrowing of the artery following angioplasty), and poor prognosis is associated with the increased number of monocytes-derived macrophages (MDMs) in the arterial wall. Local administration of a suitable delivery system targeting MDMs could provide effective fluorescent imaging while minimizing systemic exposure and toxicity. We report here on the physicochemical characteristics and the structural stability of MDMs-targeted liposomal QDs (LipQDs), cellular uptake and cytotoxicity, the systemic biodistribution of LipQDs following local intra-luminal administration of LipQDs in carotid-injured rats vs. systemic administration, and imaging of QDs in the arterial tissue. The local treatment with LipQDs was found to be a suitable approach for targeting QDs to MDMs in the injured artery. In contrast to free QDs, the LipQDs formulation exhibited unique properties including structural and fluorescent stability, increased accumulation and retention for up to 24 h, and targeting properties enabling imaging of MDMs. MDMs imaging by targeted nanoparticles (NPs) could potentially serve for the detection of MDMs density in the injured artery for diagnostic purposes.
AB - Quantum dots offer superior optical features and hold a great potential as an imaging tool in comparison to ‘conventional’ fluorescent dyes. However, in vivo application in inflammatory-associated disorders is limited due to potential toxicity following systemic administration. Vascular inflammation contributes to cardiovascular diseases such as restenosis (re-narrowing of the artery following angioplasty), and poor prognosis is associated with the increased number of monocytes-derived macrophages (MDMs) in the arterial wall. Local administration of a suitable delivery system targeting MDMs could provide effective fluorescent imaging while minimizing systemic exposure and toxicity. We report here on the physicochemical characteristics and the structural stability of MDMs-targeted liposomal QDs (LipQDs), cellular uptake and cytotoxicity, the systemic biodistribution of LipQDs following local intra-luminal administration of LipQDs in carotid-injured rats vs. systemic administration, and imaging of QDs in the arterial tissue. The local treatment with LipQDs was found to be a suitable approach for targeting QDs to MDMs in the injured artery. In contrast to free QDs, the LipQDs formulation exhibited unique properties including structural and fluorescent stability, increased accumulation and retention for up to 24 h, and targeting properties enabling imaging of MDMs. MDMs imaging by targeted nanoparticles (NPs) could potentially serve for the detection of MDMs density in the injured artery for diagnostic purposes.
KW - Fluorescent imaging
KW - Liposomes
KW - Local administration
KW - Monocytes-derived macrophages
KW - Quantum dots
KW - Vascular injury
UR - http://www.scopus.com/inward/record.url?scp=85076559543&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2019.12.009
DO - 10.1016/j.jconrel.2019.12.009
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C2 - 31830540
AN - SCOPUS:85076559543
SN - 0168-3659
VL - 318
SP - 145
EP - 157
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -