Nematostella vectensis has emerged as one as the most established models of the phylum Cnidaria (sea anemones, corals, hydroids and jellyfish) for studying animal evolution. The availability of a reference genome and the relative ease of culturing and genetically manipulating this organism make it an attractive model for addressing questions regarding the evolution of venom, development, regeneration and other interesting understudied questions. We and others have previously reported the use of tissue‐specific promoters for investigating the function of a tissue or a cell type of interest in vivo. However, to our knowledge, genetic regulators at the whole organism level have not been reported yet. Here we report the identification and utilization of a ubiquitous promoter to drive a wide and robust expression of the fluorescent protein mCherry. We generated animals containing a TATA binding protein (TBP) promoter upstream of the mCherry gene. Flow cytometry and fluorescent microscopy revealed expression of mCherry in diverse cell types, accounting for more than 90% of adult animal cells. Furthermore, we detected a stable mCherry expression at different life stages and throughout generations. This tool will expand the existing experimental toolbox to facilitate genetic engineering and functional studies at the whole organism level.
Bibliographical noteFunding Information:
Funding: This research was funded by European Research Council Consolidator grant AntiViralEvo, grant number 863809.
This research was funded by European Research Council Consolidator grant AntiViralEvo, grant number 863809. Acknowledgments: The authors are thankful to William Breuer (Interdepartmental Equipment Unit, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem) for his help with FACS and to Reuven Aharoni (Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem) for his administrative help.
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- Gene expression
- Nematostella vectensis
- Transgenic animal
- Ubiquitous promoter