Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb; Nadja Kampschulte; Gerhard Hagn; Kathrin Plitzko; Sven W. Meckelmann; Soumita Ghosh; Robin Joshi; Julia Kuligowski; Dajana Vuckovic; Marina T. Botana; Ángel Sánchez-Illana; Fereshteh Zandkarimi; Aditi Das; Jun Yang; Louis Schmidt; Antonio Checa; Helen M. Roche; Aaron M. Armando; Matthew L. Edin; Fred B. Lih; Juan J. Aristizabal-Henao; Sayuri Miyamoto; Francesca Giuffrida; Arieh Moussaieff; Rosário Domingues; Michael Rothe; Christine Hinz; Ujjalkumar Subhash Das; Katharina M. Rund; Ameer Y. Taha; Robert K. Hofstetter; Markus Werner; Oliver Werz; Astrid S. Kahnt; Justine Bertrand-Michel; Pauline Le Faouder; Robert Gurke; Dominique Thomas; Federico Torta; Ivana Milic; Irundika H.K. Dias; Corinne M. Spickett; Denise Biagini; Tommaso Lomonaco; Helena Idborg; Jun Yan Liu; Maria Fedorova; David A. Ford; Anne Barden; Trevor A. Mori; Paul D. Kennedy; Kirk Maxey; Julijana Ivanisevic; Hector Gallart-Ayala; Cécile Gladine; Markus Wenk; Jean Marie Galano; Thierry Durand; Ken D. Stark; Coral Barbas; Ulrike Garscha; Stacy L. Gelhaus; Uta Ceglarek; Nicolas Flamand; Julian L. Griffin; Robert Ahrends; Makoto Arita; Darryl C. Zeldin; Francisco J. Schopfer; Oswald Quehenberger; Randall Julian; Anna Nicolaou; Ian A. Blair; Michael P. Murphy; Bruce D. Hammock; Bruce Freeman; Gerhard Liebisch; Charles N. Serhan; Harald C. Köfeler; Per Johan Jakobsson; Dieter Steinhilber; Michael H. Gelb; Michal Holčapek; Ruth Andrew; Martin Giera; Garret A. FitzGerald; Robert C. Murphy; John W. Newman; Edward A. Dennis; Kim Ekroos; Ginger L. Milne; Miguel A. Gijón; Hubert W. Vesper; Craig E. Wheelock; Valerie B. O'Donnell

doi:10.1126/scisignal.adw1245

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb^*, Nadja Kampschulte, Gerhard Hagn, Kathrin Plitzko, Sven W. Meckelmann, Soumita Ghosh, Robin Joshi, Julia Kuligowski, Dajana Vuckovic, Marina T. Botana, Ángel Sánchez-Illana, Fereshteh Zandkarimi, Aditi Das, Jun Yang, Louis Schmidt, Antonio Checa, Helen M. Roche, Aaron M. Armando, Matthew L. Edin, Fred B. LihJuan J. Aristizabal-Henao, Sayuri Miyamoto, Francesca Giuffrida, Arieh Moussaieff, Rosário Domingues, Michael Rothe, Christine Hinz, Ujjalkumar Subhash Das, Katharina M. Rund, Ameer Y. Taha, Robert K. Hofstetter, Markus Werner, Oliver Werz, Astrid S. Kahnt, Justine Bertrand-Michel, Pauline Le Faouder, Robert Gurke, Dominique Thomas, Federico Torta, Ivana Milic, Irundika H.K. Dias, Corinne M. Spickett, Denise Biagini, Tommaso Lomonaco, Helena Idborg, Jun Yan Liu, Maria Fedorova, David A. Ford, Anne Barden, Trevor A. Mori, Paul D. Kennedy, Kirk Maxey, Julijana Ivanisevic, Hector Gallart-Ayala, Cécile Gladine, Markus Wenk, Jean Marie Galano, Thierry Durand, Ken D. Stark, Coral Barbas, Ulrike Garscha, Stacy L. Gelhaus, Uta Ceglarek, Nicolas Flamand, Julian L. Griffin, Robert Ahrends, Makoto Arita, Darryl C. Zeldin, Francisco J. Schopfer, Oswald Quehenberger, Randall Julian, Anna Nicolaou, Ian A. Blair, Michael P. Murphy, Bruce D. Hammock, Bruce Freeman, Gerhard Liebisch, Charles N. Serhan, Harald C. Köfeler, Per Johan Jakobsson, Dieter Steinhilber, Michael H. Gelb, Michal Holčapek, Ruth Andrew, Martin Giera, Garret A. FitzGerald, Robert C. Murphy, John W. Newman, Edward A. Dennis, Kim Ekroos, Ginger L. Milne, Miguel A. Gijón, Hubert W. Vesper, Craig E. Wheelock, Valerie B. O'Donnell

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

Original language	English
Pages (from-to)	eadw1245
Journal	Science Signaling
Volume	18
Issue number	887
DOIs	https://doi.org/10.1126/scisignal.adw1245
State	Published - 20 May 2025

Bibliographical note

Publisher Copyright:
Copyright © 2025 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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10.1126/scisignal.adw1245

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Schebb, N. H., Kampschulte, N., Hagn, G., Plitzko, K., Meckelmann, S. W., Ghosh, S., Joshi, R., Kuligowski, J., Vuckovic, D., Botana, M. T., Sánchez-Illana, Á., Zandkarimi, F., Das, A., Yang, J., Schmidt, L., Checa, A., Roche, H. M., Armando, A. M., Edin, M. L., ... O'Donnell, V. B. (2025). Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry. Science Signaling, 18(887), eadw1245. https://doi.org/10.1126/scisignal.adw1245

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title = "Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry",

abstract = "Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.",

author = "Schebb, \{Nils Helge\} and Nadja Kampschulte and Gerhard Hagn and Kathrin Plitzko and Meckelmann, \{Sven W.\} and Soumita Ghosh and Robin Joshi and Julia Kuligowski and Dajana Vuckovic and Botana, \{Marina T.\} and {\'A}ngel S{\'a}nchez-Illana and Fereshteh Zandkarimi and Aditi Das and Jun Yang and Louis Schmidt and Antonio Checa and Roche, \{Helen M.\} and Armando, \{Aaron M.\} and Edin, \{Matthew L.\} and Lih, \{Fred B.\} and Aristizabal-Henao, \{Juan J.\} and Sayuri Miyamoto and Francesca Giuffrida and Arieh Moussaieff and Ros{\'a}rio Domingues and Michael Rothe and Christine Hinz and Das, \{Ujjalkumar Subhash\} and Rund, \{Katharina M.\} and Taha, \{Ameer Y.\} and Hofstetter, \{Robert K.\} and Markus Werner and Oliver Werz and Kahnt, \{Astrid S.\} and Justine Bertrand-Michel and \{Le Faouder\}, Pauline and Robert Gurke and Dominique Thomas and Federico Torta and Ivana Milic and Dias, \{Irundika H.K.\} and Spickett, \{Corinne M.\} and Denise Biagini and Tommaso Lomonaco and Helena Idborg and Liu, \{Jun Yan\} and Maria Fedorova and Ford, \{David A.\} and Anne Barden and Mori, \{Trevor A.\} and Kennedy, \{Paul D.\} and Kirk Maxey and Julijana Ivanisevic and Hector Gallart-Ayala and C{\'e}cile Gladine and Markus Wenk and Galano, \{Jean Marie\} and Thierry Durand and Stark, \{Ken D.\} and Coral Barbas and Ulrike Garscha and Gelhaus, \{Stacy L.\} and Uta Ceglarek and Nicolas Flamand and Griffin, \{Julian L.\} and Robert Ahrends and Makoto Arita and Zeldin, \{Darryl C.\} and Schopfer, \{Francisco J.\} and Oswald Quehenberger and Randall Julian and Anna Nicolaou and Blair, \{Ian A.\} and Murphy, \{Michael P.\} and Hammock, \{Bruce D.\} and Bruce Freeman and Gerhard Liebisch and Serhan, \{Charles N.\} and K{\"o}feler, \{Harald C.\} and Jakobsson, \{Per Johan\} and Dieter Steinhilber and Gelb, \{Michael H.\} and Michal Hol{\v c}apek and Ruth Andrew and Martin Giera and FitzGerald, \{Garret A.\} and Murphy, \{Robert C.\} and Newman, \{John W.\} and Dennis, \{Edward A.\} and Kim Ekroos and Milne, \{Ginger L.\} and Gij{\'o}n, \{Miguel A.\} and Vesper, \{Hubert W.\} and Wheelock, \{Craig E.\} and O'Donnell, \{Valerie B.\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2025 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.",

year = "2025",

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doi = "10.1126/scisignal.adw1245",

language = "אנגלית",

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Schebb, NH, Kampschulte, N, Hagn, G, Plitzko, K, Meckelmann, SW, Ghosh, S, Joshi, R, Kuligowski, J, Vuckovic, D, Botana, MT, Sánchez-Illana, Á, Zandkarimi, F, Das, A, Yang, J, Schmidt, L, Checa, A, Roche, HM, Armando, AM, Edin, ML, Lih, FB, Aristizabal-Henao, JJ, Miyamoto, S, Giuffrida, F, Moussaieff, A, Domingues, R, Rothe, M, Hinz, C, Das, US, Rund, KM, Taha, AY, Hofstetter, RK, Werner, M, Werz, O, Kahnt, AS, Bertrand-Michel, J, Le Faouder, P, Gurke, R, Thomas, D, Torta, F, Milic, I, Dias, IHK, Spickett, CM, Biagini, D, Lomonaco, T, Idborg, H, Liu, JY, Fedorova, M, Ford, DA, Barden, A, Mori, TA, Kennedy, PD, Maxey, K, Ivanisevic, J, Gallart-Ayala, H, Gladine, C, Wenk, M, Galano, JM, Durand, T, Stark, KD, Barbas, C, Garscha, U, Gelhaus, SL, Ceglarek, U, Flamand, N, Griffin, JL, Ahrends, R, Arita, M, Zeldin, DC, Schopfer, FJ, Quehenberger, O, Julian, R, Nicolaou, A, Blair, IA, Murphy, MP, Hammock, BD, Freeman, B, Liebisch, G, Serhan, CN, Köfeler, HC, Jakobsson, PJ, Steinhilber, D, Gelb, MH, Holčapek, M, Andrew, R, Giera, M, FitzGerald, GA, Murphy, RC, Newman, JW, Dennis, EA, Ekroos, K, Milne, GL, Gijón, MA, Vesper, HW, Wheelock, CE & O'Donnell, VB 2025, 'Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry', Science Signaling, vol. 18, no. 887, pp. eadw1245. https://doi.org/10.1126/scisignal.adw1245

TY - JOUR

T1 - Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

AU - Schebb, Nils Helge

AU - Kampschulte, Nadja

AU - Hagn, Gerhard

AU - Plitzko, Kathrin

AU - Meckelmann, Sven W.

AU - Ghosh, Soumita

AU - Joshi, Robin

AU - Kuligowski, Julia

AU - Vuckovic, Dajana

AU - Botana, Marina T.

AU - Sánchez-Illana, Ángel

AU - Zandkarimi, Fereshteh

AU - Das, Aditi

AU - Yang, Jun

AU - Schmidt, Louis

AU - Checa, Antonio

AU - Roche, Helen M.

AU - Armando, Aaron M.

AU - Edin, Matthew L.

AU - Lih, Fred B.

AU - Aristizabal-Henao, Juan J.

AU - Miyamoto, Sayuri

AU - Giuffrida, Francesca

AU - Moussaieff, Arieh

AU - Domingues, Rosário

AU - Rothe, Michael

AU - Hinz, Christine

AU - Das, Ujjalkumar Subhash

AU - Rund, Katharina M.

AU - Taha, Ameer Y.

AU - Hofstetter, Robert K.

AU - Werner, Markus

AU - Werz, Oliver

AU - Kahnt, Astrid S.

AU - Bertrand-Michel, Justine

AU - Le Faouder, Pauline

AU - Gurke, Robert

AU - Thomas, Dominique

AU - Torta, Federico

AU - Milic, Ivana

AU - Dias, Irundika H.K.

AU - Spickett, Corinne M.

AU - Biagini, Denise

AU - Lomonaco, Tommaso

AU - Idborg, Helena

AU - Liu, Jun Yan

AU - Fedorova, Maria

AU - Ford, David A.

AU - Barden, Anne

AU - Mori, Trevor A.

AU - Kennedy, Paul D.

AU - Maxey, Kirk

AU - Ivanisevic, Julijana

AU - Gallart-Ayala, Hector

AU - Gladine, Cécile

AU - Wenk, Markus

AU - Galano, Jean Marie

AU - Durand, Thierry

AU - Stark, Ken D.

AU - Barbas, Coral

AU - Garscha, Ulrike

AU - Gelhaus, Stacy L.

AU - Ceglarek, Uta

AU - Flamand, Nicolas

AU - Griffin, Julian L.

AU - Ahrends, Robert

AU - Arita, Makoto

AU - Zeldin, Darryl C.

AU - Schopfer, Francisco J.

AU - Quehenberger, Oswald

AU - Julian, Randall

AU - Nicolaou, Anna

AU - Blair, Ian A.

AU - Murphy, Michael P.

AU - Hammock, Bruce D.

AU - Freeman, Bruce

AU - Liebisch, Gerhard

AU - Serhan, Charles N.

AU - Köfeler, Harald C.

AU - Jakobsson, Per Johan

AU - Steinhilber, Dieter

AU - Gelb, Michael H.

AU - Holčapek, Michal

AU - Andrew, Ruth

AU - Giera, Martin

AU - FitzGerald, Garret A.

AU - Murphy, Robert C.

AU - Newman, John W.

AU - Dennis, Edward A.

AU - Ekroos, Kim

AU - Milne, Ginger L.

AU - Gijón, Miguel A.

AU - Vesper, Hubert W.

AU - Wheelock, Craig E.

AU - O'Donnell, Valerie B.

PY - 2025/5/20

Y1 - 2025/5/20

N2 - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

AB - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

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SN - 1945-0877

VL - 18

SP - eadw1245

JO - Science Signaling

JF - Science Signaling

IS - 887

ER -

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

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