TY - JOUR
T1 - Techniques for analysis of glucose binding by human erythrocyte membranes
AU - Levine, M.
AU - Stein, W. D.
PY - 1967/9/9
Y1 - 1967/9/9
N2 - Two methods of measuring the binding of glucose by a component of the human erythrocyte membrane are described and compared, namely: (1) retardation of d-[3H]glucose with respect to l-[14C]glucose on columns of 'Celite'-ghost mixtures and (2) a new technique of ultrafiltration of a solution of d-[3H]glucose, l-[14C]-glucose and cell membrane extract. The binding is shown to be closely associated with the glucose transport system by the following criteria for the identification of a facilitated diffusion system: (a) The binding is specific for d-glucose in preference to the l form. (b) Phloretin, a competitive inhibitor of the monosaccharide transport system as studied by kinetic experiments, is shown to inhibit the binding. (c) Saturation of binding is indicated at high levels of glucose.
AB - Two methods of measuring the binding of glucose by a component of the human erythrocyte membrane are described and compared, namely: (1) retardation of d-[3H]glucose with respect to l-[14C]glucose on columns of 'Celite'-ghost mixtures and (2) a new technique of ultrafiltration of a solution of d-[3H]glucose, l-[14C]-glucose and cell membrane extract. The binding is shown to be closely associated with the glucose transport system by the following criteria for the identification of a facilitated diffusion system: (a) The binding is specific for d-glucose in preference to the l form. (b) Phloretin, a competitive inhibitor of the monosaccharide transport system as studied by kinetic experiments, is shown to inhibit the binding. (c) Saturation of binding is indicated at high levels of glucose.
UR - http://www.scopus.com/inward/record.url?scp=0014196995&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(67)90101-0
DO - 10.1016/0005-2736(67)90101-0
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C2 - 4292718
AN - SCOPUS:0014196995
SN - 0005-2736
VL - 135
SP - 710
EP - 716
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 4
ER -