Techniques for analysis of glucose binding by human erythrocyte membranes

M. Levine*, W. D. Stein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Two methods of measuring the binding of glucose by a component of the human erythrocyte membrane are described and compared, namely: (1) retardation of d-[3H]glucose with respect to l-[14C]glucose on columns of 'Celite'-ghost mixtures and (2) a new technique of ultrafiltration of a solution of d-[3H]glucose, l-[14C]-glucose and cell membrane extract. The binding is shown to be closely associated with the glucose transport system by the following criteria for the identification of a facilitated diffusion system: (a) The binding is specific for d-glucose in preference to the l form. (b) Phloretin, a competitive inhibitor of the monosaccharide transport system as studied by kinetic experiments, is shown to inhibit the binding. (c) Saturation of binding is indicated at high levels of glucose.

Original languageEnglish
Pages (from-to)710-716
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume135
Issue number4
DOIs
StatePublished - 9 Sep 1967
Externally publishedYes

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