TY - JOUR
T1 - Temperature dependence of uridine transport in quiescent and serum-stimulated 3T3 cells
AU - Stein, W. D.
AU - Rosengurt, Enrique
PY - 1976/1/8
Y1 - 1976/1/8
N2 - 1. (1) The kinetics of uptake of uridine into 3T3 cells have been measured as a function of concentration in the temperature range 5-37 °C, for both quiscent and serum-stimulated cells. 2. (2) The maximum velocity of uridine uptake is increased some ten-fold by adding serum, but the half-saturation concentration is not systematically affected in this temperature range. 3. (3) A detailed study of the temperature dependence of the maximum velocity of transport in the range 4-43 °C shows that the activation energy of uridine transport is not increased following serum activation. 4. (4) The data suggest that any change in membrane fluidity that might occur as a result of serum activation does not in itself lead to a more rapid rate of turnover of the individual uridine carriers. It would appear, rather, that there is an increase in the number of functional uridine carriers.
AB - 1. (1) The kinetics of uptake of uridine into 3T3 cells have been measured as a function of concentration in the temperature range 5-37 °C, for both quiscent and serum-stimulated cells. 2. (2) The maximum velocity of uridine uptake is increased some ten-fold by adding serum, but the half-saturation concentration is not systematically affected in this temperature range. 3. (3) A detailed study of the temperature dependence of the maximum velocity of transport in the range 4-43 °C shows that the activation energy of uridine transport is not increased following serum activation. 4. (4) The data suggest that any change in membrane fluidity that might occur as a result of serum activation does not in itself lead to a more rapid rate of turnover of the individual uridine carriers. It would appear, rather, that there is an increase in the number of functional uridine carriers.
UR - http://www.scopus.com/inward/record.url?scp=0016915575&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(76)90376-X
DO - 10.1016/0005-2736(76)90376-X
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C2 - 1244857
AN - SCOPUS:0016915575
SN - 0005-2736
VL - 419
SP - 112
EP - 118
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -